Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) UPF3 Polyclonal antibody

Uniprot No.

Q9FVW4

Target Names

UPF3

Alternative Names

UPF3 antibody; At1g33980 antibody; F12G12.20 antibody; T15K4.3 antibody; Regulator of nonsense transcripts UPF3 antibody; Nonsense mRNA reducing factor UPF3 antibody; Up-frameshift suppressor 3 homolog antibody; AtUpf3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana UPF3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. Binds spliced mRNA upstream of exon-exon junctions. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (premature termination codon PTC) by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Eliminates the production of nonsense-containing RNAs (ncRNAs). Required for plant development and adaptation to environmental stresses, including plant defense and response to wounding.

Gene References into Functions

The results demonstrate that, in contrast to mammalian cells, a delicate balance of UPF3 transcript levels by its feedback loop and by restriction of its transcription, are crucial for proper nonsense-mediated-decay regulation in Arabidopsis. PMID: 25820429
Here, we analyzed the abundances of all splice variants for each alternatively spliced gene in an Arabidopsis mutant that lacks UPF3, one of the core components of NMD machinery, to determine if the PTC-containing transcripts are degraded by NMD. PMID: 19863731
Our results indicate that the AtUPF3 gene is required by the plant nonsense-mediated mRNA decay system to obviate aberrantly spliced mRNA. PMID: 16098107
Data show that in mutants impaired in the nonsense-mediated decay (NMD) components UPF3, levels of the alternatively spliced AtGRP7 and AtGRP8 transcripts are strongly elevated, implicating UPF3 in the decay of these clock-regulated transcripts. PMID: 17924945
The nonsense-mediated decay factor UPF3 localize to the nucleolus, suggesting that the Arabidopsis nucleolus is therefore involved in identifying aberrant mRNAs and NMD. PMID: 19602621

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Subcellular Location

Nucleus, nucleolus. Nucleus. Cytoplasm.

Protein Families

RENT3 family

Database Links

KEGG: ath:AT1G33980

STRING: 3702.AT1G33980.2

UniGene: At.21064

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UPF3 Antibody

Product Details

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Target Background