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nrdF Antibody

Datasheet
Code CSB-PA336265XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) nrdF Polyclonal antibody
Uniprot No.
P37146
Target Names
nrdF
Alternative Names
nrdF antibody; ygaD antibody; b2676 antibody; JW2651 antibody; Ribonucleoside-diphosphate reductase 2 subunit beta antibody; EC 1.17.4.1 antibody; R2F protein antibody; Ribonucleotide reductase 2 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) nrdF protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Related Products

nrdF Proteins

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Target Background

Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis.
Gene References into Functions
  1. Thus NrdEF supports DNA replication when iron is unavailable to activate the housekeeping NrdAB enzyme. PMID: 21338418
  2. results strongly suggest that E. coli NrdF is an obligate manganese protein in vivo and that the Mn(III)(2)-Y(*) cofactor assembly pathway PMID: 21250660
  3. crystal structure of Mn(II)2-NrdF and Fe(II)2-NrdF reveal different coordination environments; in structures of Mn(II)2-NrdF in complex with reduced and oxidized NrdI, a continuous channel connects NrdI flavin cofactor to the NrdF Mn(II)2 active site PMID: 20688982
  4. Functional long-range proton-coupled electron transfer in ribonucleoside diphosphate reductase occurs via an optimized pathway of amino acids spanning >35 A across the alpha:beta interface. PMID: 19916558
Protein Families
Ribonucleoside diphosphate reductase small chain family
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