RPL5A Antibody

Code CSB-PA818521XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPL5A Polyclonal antibody
Uniprot No.
Target Names
RPL5A
Alternative Names
RPL5A antibody; AE6 antibody; ATL5 antibody; OLI5 antibody; PGY3 antibody; At3g25520 antibody; MWL2.17 antibody; 60S ribosomal protein L5-1 antibody; Ribosomal protein L5 A antibody; Protein ASYMMETRIC LEAVES1/2 ENHANCER 6 antibody; Protein OLIGOCELLULA 5 antibody; Protein PIGGYBACK 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPL5A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Seems involved in the regulation of cell proliferation. Essential in leaf polarity establishment, probably having a role for translation in leaf dorsoventral patterning to specify leaf adaxial identity.
Gene References into Functions
  1. The authors show that Arabidopsis thaliana L5 and TFIIIA also bind potato spindle tuber viroid (+) RNA in vitro with the same affinity as they bind 5S rRNA, whereas the affinity for a chloroplastic viroid is significantly lower. PMID: 21153748
Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.
Protein Families
Universal ribosomal protein uL18 family
Tissue Specificity
Expressed in seedlings, roots, stems, leaves, inflorescences and siliques.
Database Links

KEGG: ath:AT3G25520

STRING: 3702.AT3G25520.1

UniGene: At.23809

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