RNL Antibody

Datasheet

Code	CSB-PA175201XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RNL Polyclonal antibody

Uniprot No.

Q0WL81

Target Names

RNL

Alternative Names

RNL antibody; RLG1 antibody; At1g07910 antibody; T6D22.1 antibody; tRNA ligase 1 antibody; AtRLG1 antibody; AtRNL antibody; AtRlg1p antibody; EC 6.5.1.3 antibody; Protein AT.I.24-9 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RNL protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Essential component of stress-response pathways entailing repair of RNA breaks with 2',3'-cyclic phosphate and 5'-OH ends. Tri-functional enzyme that repairs RNA breaks with 2',3'-cyclic-PO(4) and 5'-OH ends. The ligation activity requires three sequential enzymatic activities: opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester (CPDase activity), phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP (kinase activity) and ligation of the two tRNA halves in an ATP-dependent reaction (ligase activity). Deficient in transferring AMP to pRNA(OH) to form AppRNA(OH) but proficient at sealing pre-adenylylated AppRNA(OH). CPDase and kinase reactions are almost insensitive to RNA length, whereas the ligase activity decreases with shorter RNA size. Can also splice DNA ended by a single 3'-terminal ribonucleoside 2',3'-cyclic-PO(4). Binds to mRNA, mature and immature. Exhibits tRNA ligase activity in vitro. Required for the splicing of precursor tRNA molecules containing introns. Can circularize an intron cleaved from a pre-tRNA by splicing endonuclease in vitro. Seems not involved in unfolded protein response (UPR) in the endoplasmic reticulum. Involved in auxin signaling and polar transport during organ morphogenesis.

Gene References into Functions

essential for tRNA splicing and zygote division PMID: 28631407

Subcellular Location

Nucleus. Cytoplasm.

Protein Families

TRL1 family

Tissue Specificity

Mainly expressed in proliferating cells and tissues such as root meristems, the vasculature of developing plantlets, flowers and elongating tissue.

Database Links

KEGG: ath:AT1G07910

STRING: 3702.AT1G07910.1

UniGene: At.42282

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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