RPK1 Antibody

Code CSB-PA944511XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPK1 Polyclonal antibody
Uniprot No.
Target Names
RPK1
Alternative Names
RPK1 antibody; TOAD1 antibody; At1g69270 antibody; F23O10.15 antibody; F4N2.23 antibody; Probable LRR receptor-like serine/threonine-protein kinase RPK1 antibody; EC 2.7.11.1 antibody; Protein TOADSTOOL 1 antibody; Receptor-like protein kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RPK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the main abscisic acid-mediated (ABA) signaling pathway and in early ABA perception. Together with RPK2, required for pattern formation along the radial axis (e.g. the apical embryonic domain cell types that generate cotyledon primordia), and the apical-basal axis (e.g. differentiation of the basal pole during early embryogenesis).
Gene References into Functions
  1. According to rpk1's auxin-related PIN1 defect, seedlings displayed defects in vascular tissue. Shoot apical meristem (SAM)-less seedlings variably expressed essential SAM specific genes along the hypocotyl-cotyledon structure into the cotyledon lamina. PMID: 26150008
  2. Mutations in the Arabidopsis RPK1 gene uncouple cotyledon anlagen and primordia by modulating epidermal cell shape and polarity. PMID: 24244845
  3. RPK1 causes salt sensitivity by increasing Na+ ion flux into cells under salt stress PMID: 24467897
  4. Fine mapping by local association analysis identified RECEPTOR-LIKE PROTEIN KINASE1 (RPK1), an abscisic acid-related receptor, as the most likely gene underlying REG-1. PMID: 24850864
  5. Sucrose-induced receptor kinase (RPK1) regulates a plasma membrane aquaporin in Arabidopsis. PMID: 23820729
  6. RPK1 plays different roles at different stages of development. The effect of ABA and ABA-inducible RPK1 on leaf senescence is dependent on the age of the plant. PMID: 21382977
  7. RPK1 overproduction controls reactive oxygen species homeostasis and enhances both water and oxidative stress tolerance in Arabidopsis PMID: 20089852
  8. RPK1 is involved in the main ABA signaling pathway and in early ABA perception in Arabidopsis. PMID: 15772289
  9. RPK1 and TOAD2 are primarily required to maintain central domain protoderm cell fate and that the loss of this key embryonic cell type in mutant embryos results indefects in other regions of embryo including the failure to initiate cotyledon primordia. PMID: 18158146

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in roots, stems, leaves, and flowers.
Database Links

KEGG: ath:AT1G69270

STRING: 3702.AT1G69270.1

UniGene: At.16506

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