RPM1 Antibody

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Code	CSB-PA657193XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPM1 Polyclonal antibody

Uniprot No.

Q39214

Target Names

RPM1

Alternative Names

RPM1 antibody; RPS3 antibody; At3g07040 antibody; F17A9.20 antibody; Disease resistance protein RPM1 antibody; Resistance to Pseudomonas syringae protein 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana RPM1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Disease resistance (R) protein that specifically recognizes the AvrRpm1 type III effector avirulence protein from Pseudomonas syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Acts via its interaction with RIN4, and probably triggers the plant resistance when RIN4 is phosphorylated by AvrRpm1. It is then degraded at the onset of the hypersensitive response.

Gene References into Functions

RPM1, RPS2 and RPS5 are R proteins. Here, we provide evidence that FLS2 is physically associated with all three R proteins. PMID: 21726371
RPM1 is activated at, and functions on, the plasma membrane. PMID: 21490299
Threonine phosphorylation of Arabidopsis RIN4 by Pseudomonas AvrRpm1 and AvrB activates Arabidopsis immune receptor RPM1. PMID: 21320695
Data show that a PP2C-type phosphatase (renamed PIA1) was identified that accumulates following infection by Pseudomonas syringae expressing AvrRpm1 and subsequent activation of the corresponding plant NB-LRR disease resistance protein RPM1. PMID: 19843314
Two closely related RPM1-interacting proteins, RIN2 and RIN3, are characterized in A. thaliana. PMID: 16212605
AvrB is activated inside the host cell by nucleotide binding and subsequent phosphorylation and interacts with RIN4. Aactivated AvrB, bound to RIN4, is indirectly recognized by RPM1 to initiate plant immune system function. PMID: 17397263

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Subcellular Location

Endomembrane system; Peripheral membrane protein.

Protein Families

Disease resistance NB-LRR family

Database Links

KEGG: ath:AT3G07040

STRING: 3702.AT3G07040.1

UniGene: At.40415

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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RPM1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background