RPN14 Antibody

Code CSB-PA144688XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPN14 Polyclonal antibody
Uniprot No.
Target Names
RPN14
Alternative Names
RPN14 antibody; YGL004C antibody; 26S proteasome regulatory subunit RPN14 antibody; Proteasome non-ATPase subunit 14 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RPN14 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Is not a genuine component of the 26S proteasome, but an auxiliary factor that interacts with the proteasomal ATPase of 19S regulatory particle (RP). Acts as a chaperone which regulates the highly structured assembly of the 19S regulatory particle. Involved in the substrate specificity of the 26S proteasome and is especially involved in the degradation of ubiquitinated GCN4. May contribute to the stability of the 26S proteasome in some stress conditions.
Gene References into Functions
  1. Rpn14 binds selectively to Rpt6's four-helix bundle, with surprisingly high affinity PMID: 23562395
  2. crystal structure of Rpn14 with an E384A mutation is presented in space group P2(1) at 1.6 A resolution PMID: 22691779
  3. By inspection of structural data along with genetic and biochemical data, we determined the specific residues of Rpn14 and Rpt6 for complementary charge interactions that are required for 19 S RP assembly PMID: 20236927
  4. RPN14 protein involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4p. PMID: 17499717
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
WD repeat PAAF1/RPN14 family
Database Links

KEGG: sce:YGL004C

STRING: 4932.YGL004C

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