rpoE Antibody

Code CSB-PA314854XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) rpoE Polyclonal antibody
Uniprot No.
Target Names
rpoE
Alternative Names
rpoE antibody; sigE antibody; b2573 antibody; JW2557 antibody; ECF RNA polymerase sigma-E factor antibody; RNA polymerase sigma-E factor antibody; Sigma-24 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) rpoE protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma-E controls the envelope stress response, responding to periplasmic protein stress, increased levels of periplasmic lipopolysaccharide (LPS) as well as heat shock and oxidative stress; it controls protein processing in the extracytoplasmic compartment. The 90 member regulon consists of the genes necessary for the synthesis and maintenance of both proteins and LPS of the outer membrane. Indirectly activates transcription of csrB and csrC, 2 sRNAs that antagonize translational regulator CsrA, linking envelope stress, the stringent response and the catabolite repression systems.
Gene References into Functions
  1. Mapping of 5' ends of rpoE mRNA identified five new transcriptional initiation sites (P1 to P5) located distal to Esigma(E)-regulated promoter. PMID: 27629414
  2. These results demonstrate that appropriate regulation of sigma(E) activity, ensuring that it is neither too high nor too low, is critical for envelope integrity and cell viability. PMID: 28373273
  3. This paper describes a unique mutation that enhances kinetics of the rpoE envelope stress response pathway rather than preinducing the response. PMID: 27325680
  4. In particular, Esigma(S) appears to contribute significantly to transcription of genes encoding proteins involved in LPS biosynthesis and in cell surface composition. Finally, our results highlight a direct role of Esigma(S) in the regulation of non coding RNAs PMID: 26020590
  5. RpoE regulates transcription of the defective prophage DLP12 lysis genes through interaction with QDLP12. PMID: 25998262
  6. However, membrane protein damage induced by gentamicin can be eliminated or repaired by RpoE- and Cpx-dependent mechanisms pre-emptively induced in P-starved cells. PMID: 25039943
  7. TraR increases the concentrations of periplasmic chaperones and proteases by directly activating the transcription of sigma(E)-dependent promoters. PMID: 25535270
  8. The sigma(E) response was activated in Deltahfq mutants of all strains tested, resulting from the misregulation ofouter membrane proteins, whereas the Cpx response was activated by loss of hfq in enteropathogenic strains, but not in E. coli K-12. PMID: 24628810
  9. Through employing a labile mutant periplasmic protein, AcrA(L222Q), it was determined that the rpoE3 mutation overcomes the DeltadegP phenotypes, in part, by activating a sigma(E)-dependent proteolytic pathway. PMID: 22439016
  10. Taken together, the results suggest that the cell lysis proceeds in the cascade of sigma(E) --> expression of micA and rybB --> reduction in Omp proteins --> disintegration of the outer membrane. PMID: 22251246
  11. Recognition of beta-strand motifs of sigma(E) by RseB is required for sigma(E) activity. PMID: 21908666
  12. RpoE is required for stress-induced lac amplification. The role of the sigma(E) stress response in mutagenesis is independent of SOS and DinB upregulation, cytoplasmic heat-shock- and RpoS-stress responses. PMID: 20497332
  13. Role in resistance to zinc, cadmium, and copper toxicity. PMID: 15774872
  14. hypotheses:(1)negatively charged N-terminal domain of sigma(70) is bound in polymerase;(2)rate-determining isomerization involves ejecting this domain, unmasking active site; (3)conversion to RP(o) involves coupled folding of downstream clamp of pol. PMID: 16475805
  15. Hfq modulates the sigmaE-mediated envelope stress response and the sigma32-mediated cytoplasmic stress response in Escherichia coli. PMID: 17158661
  16. the PDZ domain and loop L3 of DegS define a novel molecular switch allowing strict regulation of the sigmaE stress response system PMID: 17938245
  17. when sigma(E) is inhibited, cell envelope stress increases and envelope integrity is lost PMID: 18253509

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Subcellular Location
Cytoplasm.
Protein Families
Sigma-70 factor family, ECF subfamily
Database Links
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