Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Rspo3 Polyclonal antibody

Uniprot No.

Q2TJ95

Target Names

Rspo3

Alternative Names

Rspo3 antibody; R-spondin-3 antibody; Cabriolet antibody; Cysteine-rich and single thrombospondin domain-containing protein 1 antibody; Cristin-1 antibody; Nucleopondin antibody; Roof plate-specific spondin-3 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Rspo3 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.

Gene References into Functions

These results shed new light on the role of Rspo3 in heart development and demonstrate that LGR4 is the principal R-spondin 3 receptor in the heart. PMID: 29859889
Results identify a mechanism through which localized expression of RSPO3 induces proliferation of the coronary arteries at their stems and permits their formation. PMID: 28834739
Rspo3-LGR4 axis protects hepatocytes from hypoxia/reoxygenation injury via activating beta-catenin. PMID: 29555474
H. pylori infection increases stromal R-spondin 3 expression and expands the Axin2(+) cell pool to cause hyperproliferation and gland hyperplasia; the ability of stromal niche cells to control and adapt epithelial stem cell dynamics constitutes a sophisticated mechanism that orchestrates epithelial regeneration and maintenance of tissue integrity PMID: 28813421
We provide in vivo evidence that RSPO3 stimulates the crypt stem cell and niche compartments and drives rapid intestinal tumorigenesis. PMID: 27511199
Rspo3 function remains essential in adult life to ensure replenishment of lost cells and maintain the properties of the zona glomerulosa PMID: 27313319
Conditional deletion of Rspo3 in mice disrupts activation of central fate, demonstrating its crucial role in determining and maintaining beta-catenin-dependent zonation. PMID: 26655896
data identify endothelial RSPO3-driven non-canonical WNT/Ca(2+)/NFAT signaling as a critical maintenance pathway of the remodeling vasculature PMID: 26766444
Nkx2-5 has a role in regulating cardiac growth through modulation of Wnt signaling by R-spondin3 PMID: 25053429
Rspo3 loss of function mutation in combination with Rspo2 mutation,but not on its own, causes severe limb truncation. PMID: 22610508
Sexually dimorphic expression patterns were observed for WNT/beta-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. PMID: 21936019
Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID: 21397842
suggest a critical role for Rspo3 in the interaction between chorion and allantois in labyrinthine development PMID: 16963017
identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis PMID: 18842812

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Subcellular Location

Secreted.

Protein Families

R-spondin family

Tissue Specificity

Highly expressed in endothelial cells.

Database Links

KEGG: mmu:72780

STRING: 10090.ENSMUSP00000090287

UniGene: Mm.45191

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Rspo3 Antibody

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