SARD1 Antibody

Code CSB-PA281147XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SARD1 Polyclonal antibody
Uniprot No.
Target Names
SARD1
Alternative Names
SARD1 antibody; At1g73805 antibody; F25P22.22 antibody; Protein SAR DEFICIENT 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SARD1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator that binds DNA in a sequence-specific manner, 5'-GAAATTTTGG-3', to promote the expression of target genes. Recruited to the promoter of ICS1 and other defense-related genes (e.g. PR1 and SID2) in response to both biotic (e.g. Pseudomonas syringae pv. maculicola ES4326) and abiotic stresses (e.g. UV-B), thus triggering slow defense responses by stimulating salicylic acid (SA) biosynthesis. Required for basal and systemic acquired resistance to P. syringae pv. maculicola and Hyaloperonospora arabidopsidis.
Gene References into Functions
  1. Putative components of the CBP60g SARD1 signalling network have been uncovered by co-expression analysis. In an electrophoretic mobility shift assay CBP60g and SARD1 were shown to bind specifically to a 10mer oligonucleotide with the sequence GAAATTTTGG. [SARD1] PMID: 23153277
  2. SARD1 plays an important role during the defense response and does not bind calmodulin. [SARD1] PMID: 21615571
Subcellular Location
Nucleus.
Protein Families
Plant ACBP60 protein family
Database Links

KEGG: ath:AT1G73805

STRING: 3702.AT1G73805.1

UniGene: At.17985

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