SDE3 Antibody

Datasheet

Code	CSB-PA815441XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SDE3 Polyclonal antibody

Uniprot No.

Q8GYD9

Target Names

SDE3

Alternative Names

SDE3 antibody; At1g05460 antibody; T25N20.11 antibody; T25N20.26 antibody; Probable RNA helicase SDE3 antibody; EC 3.6.4.13 antibody; Silencing defective protein 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SDE3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Probable RNA helicase required for post-transcriptional gene silencing (PTGS), a process that provides protection in plants against virus infection and can suppress expression of transgenes. Plays a central role in RNA interference (RNAi) process, a process that mediates mRNA destruction of translational repression. Required for the assembly of the RISC complex, a complex required for target RNA destruction or repression. May be required in the RISC assembly to unwind miRNAs, in the production of single-stranded miRNA from the double-stranded miRNA, a key step in RISC formation. Involved in the amplification of sense-PTGS (S-PTGS), leading to siRNA production. Required for the maintenance but not the initiation of tobacco rattle virus (TRV)-mediated silencing, probably by mediating/maintaining DNA methylation and chromatin-based transcriptional gene silencing at some genomic locations.

Gene References into Functions

SDE3 is present with the PTGS effectors AGO1 and AGO2 in protein complexes with a GW-repeat-containing C-terminal domain. Contribution of RNA-helicase activity and role for AGO binding in SDE3 action, which occurs downstream of RDR6 PMID: 22940249

Subcellular Location

Cytoplasm.

Protein Families

DNA2/NAM7 helicase family, SDE3 subfamily

Database Links

KEGG: ath:AT1G05460

STRING: 3702.AT1G05460.1

UniGene: At.15329

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SDE3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background