SHR Antibody

Code CSB-PA866031XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SHR Polyclonal antibody
Uniprot No.
Target Names
SHR
Alternative Names
SHR antibody; SGR7 antibody; At4g37650 antibody; F19F18.140 antibody; Protein SHORT-ROOT antibody; AtSHR antibody; GRAS family protein 26 antibody; AtGRAS-26 antibody; Protein SHOOT GRAVITROPISM 7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana SHR protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for both cell division and cell specification. Regulates the radial organization of the shoot axial organs and is required for normal shoot gravitropism. Directly controls the transcription of SCR, and when associated with SCR, of MGP, RLK, TRI, NUC and SCL3.
Gene References into Functions
  1. This study showed that INDETERMINATE DOMAIN PROTEIN (ID) binding sequences have a crucial role in the regulation of SCARECROW and SHORT-ROOT expression.[SHR] PMID: 28324206
  2. interaction of KinG with SHR allows for the formation of stable movement complexes that facilitate the cell-to-cell transport of SHR. PMID: 29122988
  3. Arabidopsis thaliana SHORT ROOT protein SHR gene, rice OsSHR1 and OsSHR2, could complement the A. thaliana shr mutant. PMID: 28263767
  4. all of the SHR proteins function as mobile signals PMID: 25352666
  5. The results together suggest that SHR regulates vascular patterning, but not root apical meristematic activity, through cytokinin homeostasis. PMID: 22476466
  6. The SHORT-ROOT protein acts as a mobile, dose-dependent signal in patterning the ground tissue. PMID: 22826238
  7. SHR functions in plant growth and development as a regulator of cell division and meristem activity not only in the roots but also in the shoots. PMID: 22194939
  8. One mechanism by which SHR controls vascular patterning is the regulation of cytokinin homeostasis. PMID: 21951467
  9. SHR regulates a wide array of Arabidopsis root-related developmental processes. PMID: 21030506
  10. SHR plays a central role in the root vascular system to control patterning processes, possibly regulated by longitudinal and radial signals. PMID: 20680487
  11. SHR regulates the spatiotemporal activation of specific genes involved in cell division PMID: 20596025
  12. The role of SHORT-ROOT and SCARECROW proteins in the maturation of A. thaliana roots, including cell organization, is reported. PMID: 15955927
  13. Combined analysis of global expression profiles of A. thaliana after modulating SHR activity in order to identify the direct targets of SHR is reported. PMID: 16640459
  14. SHR movement from the stele is required for proper patterning of the root. Multiple domains are required for SHR movement and for proper sub-cellular localization. PMID: 19000160

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Subcellular Location
Cytoplasm. Nucleus. Early endosome. Late endosome. Recycling endosome.
Protein Families
GRAS family
Tissue Specificity
Expressed in the stele and the quiescent center. Not detected in the ground tissue cell lineage. The SHR protein moves from the stele to a single layer of adjacent cells, where it enters the nucleus.
Database Links

KEGG: ath:AT4G37650

STRING: 3702.AT4G37650.1

UniGene: At.2816

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