SOT12 Antibody

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Code	CSB-PA347147XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SOT12 Polyclonal antibody

Uniprot No.

P52839

Target Names

SOT12

Alternative Names

SOT12 antibody; RAR047 antibody; ST1 antibody; At2g03760 antibody; F19B11.21 antibody; Cytosolic sulfotransferase 12 antibody; AtSOT12 antibody; EC 2.8.2.- antibody; Sulfotransferase 1 antibody; AtST1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SOT12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24-epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR).

Gene References into Functions

AtSOT12 could sulphonate the bacterial-produced toxin cycloheximide. PMID: 25736839
Data indicate tht 7-hydroxyflavone was sulfated by all three enzymes AtSULT202E1 (At2g03770), AtSULT202B1 (At3g45070), and AtSULT202A1 (At2g03760). PMID: 23611783
Salycylic acid can be sulfonated by SOT12. Over-expressing plants are more resistant to pathogen infection. PMID: 20374532
crystal structure of At2g03760; it has high structural similarity to two human steroid sulfotransferases: human estrogen sulfotransferase and dehydroepiandrosterone sulfotransferase PMID: 15317023

Subcellular Location

Cytoplasm.

Protein Families

Sulfotransferase 1 family

Tissue Specificity

Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques.

Database Links

KEGG: ath:AT2G03760

STRING: 3702.AT2G03760.1

UniGene: At.400

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SOT12 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background