Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) SYT1 Polyclonal antibody

Uniprot No.

Q9SKR2

Target Names

SYT1

Alternative Names

SYT1 antibody; SYTA antibody; At2g20990 antibody; F26H11.25 antibody; F5H14.5 antibody; Synaptotagmin-1 antibody; NTMC2T1.1 antibody; Synaptotagmin A antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana SYT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays an important role in maintaining plasma membrane integrity during freezing and osmotic stresses. May function in membrane resealing during calcium-dependent freezing tolerance. May regulate endocytosis and endosome recycling at the plasma membrane and cell-to-cell trafficking of cabbage leaf curl virus (CaLCuV) and tobacco mosaic virus (TMV) movement proteins via plasmodesmata.

Gene References into Functions

maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum-plasma membrane contact sites PMID: 27811083
SYT1 also regulates the atypical peroxisomal myrosinase PEN2-associated secretory pathway. PMID: 27016097
The findings show that SYTA is essential to form ER-plasma membrane contact sites and suggest that plant virus movement proteins interact with SYTA to recruit these sites to alter plasmodesmata for virus cell-to-cell movement. PMID: 26166780
The subcellular localization and functional characterization of SYT1 highlights a putative role of plant endoplasmic reticulum (ER)-plasma membrane contact site components in the cellular adaptation to environmental stresses. PMID: 25792253
localization of SYT1 to the plasma membrane PMID: 20498364
Sudies show that SYTA regulates endocytosis, and suggest that distinct virus movement proteins transport their cargos to plasmodesmata for cell-to-cell spread via an endocytic recycling pathway. PMID: 20133785
Studies show that Ca2+ activates the SYT1-C2A domain, which, after binding to SNAREs, triggers vesicle-plasma-membrane fusion. PMID: 19819752
SYTA regulates plasma membrane endocytosis resulting in roles for SYTA in biotic (cell-cell movement of viruses) and abiotic stresses. SYTA function points to the importance of endocytic recycling in plant cell-cell communication via plasmodesmata. [SYTA] PMID: 20133785
The analyses support that membrane trafficking mediated by SYT1 is important for plasma membrane integrity and plant fitness. PMID: 19088329
The findings suggest that calcium-dependent freezing tolerance results from membrane resealing and that this mechanism involves SYT1 function. PMID: 19088330
Analysis of calcium-dependent freezing tolerance in Arabidopsis thaliana, indicates that this protein is involved in the repair of plasma membrane damage caused by mechanical stress. [SYT1] PMID: 19088330
A null mutant (syt1-2) at this locus in Arabidopsis thaliana results in a salt-hypersensitive phenotype and implicates the encoded protein as a required participant in the process of plasma membrane repair. [SYT1] [Synaptotagmin 1] PMID: 19088329

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Subcellular Location

Cell membrane; Single-pass membrane protein; Cytoplasmic side. Endosome membrane; Single-pass membrane protein.

Protein Families

Synaptotagmin family

Tissue Specificity

Expressed in roots, shoots, rosette and cauline leaves, inflorescences, and siliques. In roots, expressed in vascular bundle, epidermis, the differential zone of the tips of root hairs, and the quiescent center and columella of root tips.

Database Links

KEGG: ath:AT2G20990

UniGene: At.24916

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SYT1 Antibody

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Target Background