16 Antibody

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Code	CSB-PA323503XA01EDZ
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) 16 Polyclonal antibody

Uniprot No.

P17311

Target Names

Alternative Names

16 antibody; Terminase antibody; small subunit antibody; Gene product 16 antibody; gp16 antibody; Packaging protein Gp16 antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T4 (Bacteriophage T4)

Immunogen

Recombinant Enterobacteria phage T4 (Bacteriophage T4) 16 protein

Immunogen Species

Enterobacteria phage T4 (Bacteriophage T4)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

The terminase small subunit binds to the packaging initiation site and regulates the ATPase activity of the terminase large subunit. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by neck proteins.

Gene References into Functions

A packaging model in which the gp16 Coiled coil Motif is implicated in the regulation of packaging initiation and assembly of a supramolecular DNA packaging machine on the viral concatemer. PMID: 16513134

Database Links

KEGG: vg:1258811

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16 Antibody

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