TGA2 Antibody

Code CSB-PA337365XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TGA2 Polyclonal antibody
Uniprot No.
Target Names
TGA2
Alternative Names
TGA2 antibody; BZIP20 antibody; HBP1B antibody; At5g06950 antibody; MOJ9.12 antibody; Transcription factor TGA2 antibody; HBP-1b homolog antibody; AHBP-1b antibody; bZIP transcription factor 20 antibody; AtbZIP20 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TGA2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. Required to induce the systemic acquired resistance (SAR) via the regulation of pathogenesis-related genes expression. Binding to the as-1 element of PR-1 promoter is salicylic acid-inducible and mediated by NPR1. Could also bind to the C-boxes (5'-ATGACGTCAT-3') with high affinity.
Gene References into Functions
  1. Our study showed that TGA1 and TGA4 regulate Pip and SA biosynthesis by modulating the expression of SARD1 and CBP60g. PMID: 28898429
  2. AtNPR1, AtGA2, and AtPR-5, encoding specific components involved in salicylic acid regulation, synthesis, and signaling, are overexpressed in soybean roots, resistance to soybean cyst nematode is enhanced. PMID: 24739302
  3. TGA2-binding motif is directly involved in the modulation of salicylic acid-induced NIMIN-1 expression in Arabidopsis. PMID: 20198573
  4. We confirm that the NPR1 BTB/POZ domain interacts with and negates the molecular function of the TGA2 repression domain by excluding TGA2 oligomers from cognate DNA. PMID: 19915088
  5. NPR1 may transiently interact with the DNA unbound fraction of TGA2 to promote its recruitment to an active form on cognate target promoters. PMID: 18571510

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Subcellular Location
Nucleus.
Protein Families
BZIP family
Tissue Specificity
Expressed in the whole plant.
Database Links

KEGG: ath:AT5G06950

STRING: 3702.AT5G06950.1

UniGene: At.23884

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