Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) THF1 Polyclonal antibody

Uniprot No.

Q9SKT0

Target Names

THF1

Alternative Names

THF1 antibody; At2g20890 antibody; F5H14.14 antibody; Protein THYLAKOID FORMATION 1 antibody; chloroplastic antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana THF1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in a dynamic process of vesicle-mediated thylakoid membrane biogenesis. Required for the normal organization of vesicles into mature thylakoid stacks and ultimately for leaf development. Also involved in a sugar-signaling mechanism in roots by mediating signaling between the plasma membrane and the plastid. Probably acts downstream of the plasma membrane-delimited heterotrimeric G-protein GPA1 in a D-glucose signaling pathway.

Gene References into Functions

These results suggest that HCF106 and THF1 form a complex to modulate chloroplast function. PMID: 27744298
Downregulating the expression of specific plastid ribosomal proteins suppresses thf1 leaf variegation, and provide new insights into a role of THF1 in plastid gene expression. PMID: 25733183
thf1 variegation was suppressed by a mutation in sigma-FACTOR6. PMID: 25999408
conclude that the elevated anthocyanin accumulation in thf1 is attributed to an increase in JA levels PMID: 24467527
The virescent clpR4-3 mutation suppresses leaf variegation of thf1 and var2. PMID: 24124904
THF1 is required for dynamics of photosystem II-light-harvesting complexes supramolecular organization in higher plants. PMID: 23671330
Results also indicate that chlorophyll biosynthesis and expression of plastidic genes are coordinately suppressed in variegation mutant thylakoid formation1 . PMID: 21981015
The Thf1 gene product plays a crucial role in a dynamic process of vesicle-mediated thylakoid membrane biogenesis. [Thf1] PMID: 15516501
THYLAKOID FORMATION1 (THF1) is demonstrated in vivo as a Galpha interaction partner that functions downstream of the plasma membrane-delimited heterotrimeric G-protein (GPA1) in a D-glucose signaling pathway. PMID: 16582010
THF1 mutation leads to a decrease in FtsH protease that is partially recovered by the ectopic expression of GPA1. PMID: 19228339

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Subcellular Location

Plastid, chloroplast outer membrane; Single-pass membrane protein. Plastid, chloroplast stroma.

Protein Families

THF1 family

Tissue Specificity

Ubiquitous. Present at higher level in hypocotyls (at protein level). Ubiquitously expressed in all organs, in roots of both light-grown and dark-grown seedlings. Highly expressed in the root apical meristems.

Database Links

KEGG: ath:AT2G20890

STRING: 3702.AT2G20890.1

UniGene: At.46381

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THF1 Antibody

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