Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TOP2 Polyclonal antibody

Uniprot No.

P06786

Target Names

TOP2

Alternative Names

TOP2 antibody; TOR3 antibody; YNL088W antibody; N2244 antibody; DNA topoisomerase 2 antibody; EC 5.6.2.2 antibody; DNA topoisomerase II antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TOP2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Gene References into Functions

Condensin DNA supercoiling activity correlates with Top2 intertwine removal during DNA replication. PMID: 27716481
Topoisomerase II plays a central role in a molecular pathway for crossover interference PMID: 25043020
Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils. PMID: 24859967
the cell cycle delay depends on the divergent and catalytically inert C-terminal region of Topo II, indicating that transmission of a checkpoint signal may occur via the C-terminus PMID: 24098144
Structure of topoisomerase II-DNA-nucleotide complex produces an unexpected interaction between DNA and a conformational transducing element in the ATPase domain, which is critical for both DNA-stimulated ATP hydrolysis and global topoisomerase activity. PMID: 23022727
a topological change on DNA drives topoisomerase II to decatenate molecules during mitosis, potentially driving the full decatenation of the genome PMID: 21393545
In eukaryotes, replication fork barriers, Rrm3, and Top2 coordinate replication fork progression and fusion at chromosomal termination regions, thus counteracting abnormal genomic transitions. PMID: 20797631
structure of the DNA-binding and cleavage core of Saccharomyces cerevisiae topoisomerase II covalently linked to DNA through its active-site tyrosine at 2.5A resolution PMID: 20485342
compromised Topo II function activates a yeast checkpoint system that operates by a novel mechanism PMID: 16651657
structure of a complex between the DNA-binding and cleavage core of Saccharomyces cerevisiae Topo II (also known as Top2) and a gate-DNA segment PMID: 18097402
kinetic and structural analysis of coupling between ATP binding and DNA cleavage by DNA topoisomerase II PMID: 18403371
Top2 seems to play a novel role in centromeric chromatin compaction that is distinct from decatenation PMID: 18701701
analysis of Top2 covalent complexes containing DNA single strand breaks PMID: 18723844

Hide All

Subcellular Location

Nucleus.

Protein Families

Type II topoisomerase family

Database Links

KEGG: sce:YNL088W

STRING: 4932.YNL088W

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TOP2 Antibody

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