TOR Antibody

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Code	CSB-PA861872XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TOR Polyclonal antibody

Uniprot No.

Q9FR53

Target Names

TOR

Alternative Names

TOR antibody; At1g50030 antibody; F2J10.19 antibody; Serine/threonine-protein kinase TOR antibody; EC 2.7.11.1 antibody; Protein TARGET OF RAPAMYCIN antibody; AtTOR antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TOR protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Essential cell growth regulator that controls development from early embryo to seed production. Controls plant growth in environmental stress conditions. Acts through the phosphorylation of downstream effectors that are recruited by the binding partner RAPTOR. Acts by activating transcription, protein synthesis and ribosome biogenesis, and inhibiting mRNA degradation and autophagy. Can phosphorylate TAP46, a regulatory subunit of protein phosphatase 2A that modulates cell growth and survival. Involved in modulating the transition from heterotrophic to photoautotrophic growth by regulating the expression of chloroplast- and photosynthesis-associated genes. Essential for auxin signaling transduction, probably acting in polysomes to maintain the active ATPK1/S6K1 (and thus TIF3H1/eIF3h) phosphorylation status that is critical for translation reinitiation (e.g. uORF-mRNAs loading). Promotes abscisic acid (ABA) biosynthesis. Involved in the regulation of sugar-mediated (e.g. glucose and sucrose) glycolysis- and mitochondrial bioenergetics-dependent root growth promotion. Required for sugar (e.g. glucose) promotion of hypocotyl elongation in the dark, by activating the brassinosteroid pathway and stabilizing BZR1. The regulation of BZR1 degradation is dependent on autophagy. Regulates the expression, phosphorylation and ribosome association of MRFs (e.g. MRF1, MRF3 and MRF4), especially under energy-deficient conditions.; (Microbial infection) Binding to cauliflower mosaic virus (CaMV) Tav protein is critical for both translation reinitiation and viral fitness. When activated by CaMV P6, promotes CaMV translation by inhibiting cellular autophagy and suppressing both silencing and innate immunity, thus confering sensitivity to P.syringae.; (Microbial infection) Required during infection by some potyvirus such as Watermelon mosaic virus (WMV) but not for turnip mosaic virus (TuMV).

Gene References into Functions

Sugar-activated TOR kinase phosphorylates and stabilizes E2Fa proteins to transcriptionally activate S-phase genes during callus formation. PMID: 28623850
The dependence of potyviruses on active Arabidopsis TOR signalling was different. PMID: 25979731
These findings advance the current understanding of rapamycin-TOR signaling in plants. PMID: 23989449
highlight the possible role of TOR in carbon utilization and growth regulation in Arabidopsis PMID: 23761348
TOR protein regulates growth and also influences nutrient partitioning and central energy metabolism. PMID: 23173928
systems, cellular and genetic analyses uncover TOR phosphorylation of E2Fa transcription factor for an unconventional activation of S-phase genes, and glucose-signalling defects in e2fa root meristems PMID: 23542588
Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. PMID: 22134914
The authors show that the function of Cauliflower mosaic virus viroplasmin in reinitiation depends on physical association with TOR, with viroplasmin-TOR binding being critical for both translation reinitiation and viral fitness. PMID: 21343906
TOR controls the embryogenesis, postembryonic development, and 45S rRNA production through its kinase domain in Arabidopsis. PMID: 21266656
Results provide evidence that TOR is a negative regulator of autophagy in plants. PMID: 20686696
The production of rapamycin-sensitive plants through the expression of baker's yeast FKBP12 protein illustrates the conservation of the TOR pathway in Arabidopsis. PMID: 17543119
The AtTOR kinase is one of the contributors to the link between environmental cues and growth processes in plants. PMID: 17721444

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

PI3/PI4-kinase family

Tissue Specificity

Highly expressed in root meristems, shoot apical meristem (SAM) and floral buds.

Database Links

KEGG: ath:AT1G50030

STRING: 3702.AT1G50030.1

UniGene: At.16944

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TOR Antibody

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