TPX2 Antibody

Datasheet

Code	CSB-PA150170XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TPX2 Polyclonal antibody

Uniprot No.

F4I2H7

Target Names

TPX2

Alternative Names

TPX2 antibody; At1g03780 antibody; F11M21.29 antibody; Protein TPX2 antibody; AtTPX2 antibody; Targeting protein for XKLP2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TPX2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Regulates prospindle assembly during late prophase and at the onset of mitosis, before nuclear envelope breakdown (NEB). Is exported from the nucleus shortly before NEB and organized into two polar crescents. After NEB, is progressively associated with the forming spindle. Probably mediates AUR1 activation and localization to spindle microtubules. Has a microtubule binding capability and is able to trigger microtubule assembly induced by RanGTP in a heterologous system. Not involved in phragmoplast assembly, nuclear envelope reformation, and cortical microtubule assembly at the onset of G1. Involved in the formation of specific nuclear and perinuclear microtubular arrays in the nuclei of acentrosomal plant cells. Fungi and plants have acentrosomal microtubule arrays because they lack centrosomes. They use other microtubule organizing center (MTOC) structures to organize their microtubules. May function through interaction with importin.

Gene References into Functions

Overexpressed AtTPX2 reinforces microtubule formation in the vicinity of chromatin and the nuclear envelope. PMID: 24006426
show that the Targeting Protein for Xklp2 (TPX2) protein co-localizes with microtubules on the surface of prophase nuclei of the mutants, probably participating in multipolar spindle assembly PMID: 22301959
TPX2 is localized to the cell nucleus and microtubules, and has a role in proper spindle assembly. PMID: 18941054

Subcellular Location

Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Located in the nucleoplasm during interphase until late G2, in the perinuclear region during early prophase, and at the spindle during mitosis. Localized on a specific subset of phragmoplast microtubules in early telophase.

Protein Families

TPX2 family

Database Links

KEGG: ath:AT1G03780

STRING: 3702.AT1G03780.3

UniGene: At.42523

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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