wash Antibody

Datasheet

Code	CSB-PA025972XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) wash Polyclonal antibody

Uniprot No.

Q7JW27

Target Names

wash

Alternative Names

wash antibody; CG13176WASH complex subunit 1 antibody; Protein washout antibody; WAS protein family homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) wash protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Acts as a nucleation-promoting factor by activating the Arp2/3 complex to induce actin polymerization. Participates in both linear- and branched-actin networks. Functions in linear-filament (bundled F-actin) by acting downstream of Rho1 and regulating actin and microtubule organization during oogenesis. Nucleates actin in an Arp2/3-dependent manner and exhibits F-actin and microtubule bundling and cross-linking activity in the egg chamber. During embryogenesis, acts downstream of Rho1 to activate the Arp2/3 complex which is necessary for the developmental migration of tail hemocytes anteriorly along the ventral midline. Its function in hemocyte transmigration is independent of the WASH complex. May play a role in endosomal sorting; its assembly in the WASH complex may regulate its nucleation-promoting factor (NPF) activity.

Gene References into Functions

Drosophila WASH has conserved functions in integrin receptor recycling and lysosome neutralization. PMID: 27884932
reveal a novel role for Wash in modulating nucleus morphology and in the organization of both chromatin and non-chromatin nuclear sub-structures PMID: 25754639
Developmental hemocyte migration requires the interaction of Rho1 with its downstream effector Wash, a Wiskott-Aldrich syndrome family protein. PMID: 25739458
Results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures. PMID: 19633175

Protein Families

WASH1 family

Database Links

KEGG: dme:Dmel_CG13176

STRING: 7227.FBpp0087097

UniGene: Dm.37984

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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wash Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background