XYLT Antibody

Code CSB-PA873245XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) XYLT Polyclonal antibody
Uniprot No.
Target Names
XYLT
Alternative Names
XYLT antibody; At5g55500 antibody; MTE17.21 antibody; Beta-1,2-xylosyltransferase antibody; AtXYLT antibody; EC 2.4.2.38 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana XYLT protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Glycosyltransferase involved in the xylosylation of N-glycans. Possesses beta-1,2-xylosyltransferase activity, transferring xylose from UDP-xylose to the core beta-linked mannose of N-glycans. Involved in the biosynthesis of glycoprotein bound N-glycans. Does not require metal ions for its activity.
Gene References into Functions
  1. AtXYLT acts on protein-bound N-glycans prior to alpha1,3-fucosyltransferase and mannosidase II. PMID: 22024534
  2. The substrate specificity of XylT permits the enzyme to act at multiple stages of the plant N-glycosylation pathway. PMID: 15686448
  3. Data show that expression of a hybrid enzyme of Arabidopsis thaliana xylosyltransferase and human beta-1,4-galactosyltransferase I in tobacco causes a reduction of N-glycans with potentially immunogenic core-bound xylose and fucose residues. PMID: 16675551
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Database Links

KEGG: ath:AT5G55500

STRING: 3702.AT5G55500.1

UniGene: At.1454

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