Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans zfp-1 Polyclonal antibody

Uniprot No.

P34447

Target Names

zfp-1

Alternative Names

zfp-1 antibody; F54F2.2Zinc finger protein zfp-1 antibody; Protein AF-10 homolog antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans zfp-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Recruits the histone methyltransferase dot-1.1 to chromatin to methylate 'Lys-79' of histone H3 and activate transcription. Recognizes and binds histone H3 methylated at 'Lys-4' (H3K4me) at the promoters of target genes. During stress, the zfp-1-dot-1.1 complex also plays a role in the deubiquitination of histone H2B sites, which negatively modulates the RNA polymerase II-induced transcription of highly expressed genes. In response to stress, binds to the pdk-1 promoter to negatively regulate pdk-1 expression, which negatively modulates daf-16/FOXO-mediated gene expression. Thus, most likely via this mechanism, in response to stress, it confers a protective role against neuronal necrosis. Plays a role in Insulin/IGF-1-like signaling (IIS)- and diet restriction-mediated lifespan extension by controlling daf-16/FOXO and pha-4/FOXA recruitment to target promoters. May negatively regulate the expression of genes required for vulval development. May play a role in axon guidance in D-type motor neurons. May suppress sensitivity to RNAi.; Required for migration of HSN motor neurons during embryogenesis.

Gene References into Functions

the coordinated activities of DAF-16, ZFP-1, and endogenous RNAi contribute to gene regulation during development to ensure proper neuronal positioning PMID: 24558261
ZFP-1 localization peaks overlap with H3K4 methylation-enriched promoters of actively expressed genes genomewide and that H3K4 methylation is important for ZFP-1 localization to promoters in the embryo. PMID: 23263989
We found that the function of ZFP-1 in modulating pdk-1 transcription was important for the extended lifespan of the age-1(hx546) reduction-of-function PI3 kinase mutant. PMID: 21980302

Subcellular Location

Nucleus. Chromosome.

Tissue Specificity

Isoform a: Expressed at high levels in maturing oocytes, but at low levels in the rest of the germ line (at protein level). Isoform a: Not expressed in the pharynx, germ line and tail. Isoform c: Not expressed in the germ line (at protein level). Isoform

Database Links

STRING: 6239.F54F2.2a.3

UniGene: Cel.22669

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zfp-1 Antibody

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