YDA Antibody

Code CSB-PA111421XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) YDA Polyclonal antibody
Uniprot No.
Target Names
YDA
Alternative Names
YDA antibody; At1g63700 antibody; F24D7.11 antibody; Mitogen-activated protein kinase kinase kinase YODA antibody; EC 2.7.11.25 antibody; YODA MAPKK kinase antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana YDA protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions in a MAP kinase cascade that acts as a molecular switch to regulate the first cell fate decisions in the zygote and the early embryo. Promotes elongation of the zygote and development of its basal daughter cell into the extra-embryonic suspensor. In stomatal development, acts downstream of the LRR receptor TMM, but upstream of the MKK4/MKK5-MPK3/MPK6 module to regulate stomatal cell fate before the guard mother cell (GMC) is specified. Plays a central role in both guard cell identity and pattern formation. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. Upon brassinosteroid signaling, is inhibited by phosphorylation of its auto-inhibitory N-terminal domain by the GSK3-like kinase ASK7.
Gene References into Functions
  1. We find that YODA genes promote normal stomatal spacing patterns in both Arabidopsis and Brachypodium, despite species-specific differences in those patterns. PMID: 29945871
Subcellular Location
Cytoplasm, cell cortex. Cell membrane.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Tissue Specificity
Expressed in roots, leaves, guard cells, stems, flowers and siliques.
Database Links

KEGG: ath:AT1G63700

STRING: 3702.AT1G63700.1

UniGene: At.21875

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