zif-1 Antibody

Code CSB-PA327331XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans zif-1 Polyclonal antibody
Uniprot No.
Target Names
zif-1
Alternative Names
zif-1 antibody; tag-6 antibody; F59B2.6Zinc finger-interacting factor 1 antibody; Protein zif-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans zif-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable substrate recognition component of a CBC (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ensures germline precursor cell asymmetry by targeting germline proteins for destruction if expressed in non-germline cells. This process also requires elc-1, cul-2, rbx-1 and ubc-2.
Gene References into Functions
  1. It was shown that ZIF-1 expression in embryos is regulated by five maternally-supplied RNA-binding proteins. POS-1, MEX-3, and SPN-4 function as repressors of ZIF-1 expression, whereas MEX-5 and MEX-6 antagonize this repression. PMID: 22265679
  2. zif-1 encodes the substrate-binding subunit of the E3 ligase for PIE-1 degradation during germline specification. PMID: 20826530
  3. results show that five CCCH finger proteins, components of the Caenorhabditis elegans germ plasm, are targeted for degradation by the novel CCCH-finger-binding protein ZIF-1 [ZIF-1] PMID: 12894212
Database Links

KEGG: cel:CELE_F59B2.6

STRING: 6239.F59B2.6

UniGene: Cel.38655

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