Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans zyg-1 Polyclonal antibody

Uniprot No.

Q9GT24

Target Names

zyg-1

Alternative Names

zyg-1 antibody; F59E12.2Probable serine/threonine-protein kinase zyg-1 antibody; EC 2.7.11.1 antibody; Zygote defective protein 1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans zyg-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Protein kinase that plays a central role in centrosome duplication, control of centrosome size, spindle formation and nuclear envelope breakdown during cell divisions. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome. Its role in nuclear envelope breakdown is mediated by the spindly-like protein spdl-1 and the RZZ complex, which in turn recruits the spindle checkpoint proteins mdf-1 and mdf-2, dynein and dynactin to unattached kinetochores.

Gene References into Functions

These findings lead us to conclude that ZYG-1 promotes limited centriole amplification solely during the symmetric division in the C. elegans seam lineage. PMID: 29307730
Structure of the C. elegans ZYG-1 cryptic polo box suggests a conserved mechanism for centriolar docking of Plk4 kinases. PMID: 24980795
Direct interactions of the SAS-6 coiled coil with ZYG-1 and SAS-5 are required to recruit SAS-6 to the mother centriole. PMID: 23673331
Data show that levels of the C. elegans Plk4 homolog ZYG-1 are elevated by impairing proteasome or SCF function, indicating that ZYG-1 is regulated by a conserved mechanism. PMID: 22623721
Data reveal differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, and reveal an important role for centrosomes in spermatid formation. PMID: 20144993
Study demonstrates that the kinase ZYG-1 phosphorylates the coiled-coil protein SAS-6 at serine 123 in vitro; this phosphorylation event is crucial for centriole formation in vivo. PMID: 20059959
Numerous candidate regulators of centrosome duplication and regulatory mechanism involving factors that tether the centrosome to the nucleus, have been identified. PMID: 17446307
SZY-20 opposes the Plk4-related kinase ZYG-1 to limit centrosome size PMID: 19081077

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Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Component of the centrosome (PubMed:11371350). Present at centrioles only immediately before their duplication (PubMed:11371350). Expression at centrosomes increases from prophase to anaphase during mitosis (PubMed:22623721).

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Database Links

KEGG: cel:CELE_F59E12.2

STRING: 6239.F59E12.2.2

UniGene: Cel.19371

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zyg-1 Antibody

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