Target Name
Cardiac Troponin Ⅰ(cTn-Ⅰ)
Alternative Names
TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 95 | |
Range % | 90-100 | |
1:2 | Average % | 89 | |
Range % | 85-99 | |
1:4 | Average % | 98 | |
Range % | 91-104 | |
1:8 | Average % | 96 | |
Range % | 91-100 | |
Recovery
The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 97 | 92-108 | |
EDTA plasma (n=4) | 95 | 90-101 | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
pg/ml | OD1 | OD2 | Average | Corrected | | 1500 | 2.245 | 2.250 | 2.248 | 2.071 | | 750 | 1.716 | 1.704 | 1.710 | 1.533 | | 375 | 1.144 | 1.161 | 1.153 | 0.976 | | 187.5 | 0.790 | 0.775 | 0.783 | 0.606 | | 94 | 0.459 | 0.474 | 0.467 | 0.290 | | 47 | 0.305 | 0.289 | 0.297 | 0.120 | | 23.5 | 0.215 | 0.211 | 0.213 | 0.036 | | 0 | 0.176 | 0.177 | 0.177 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog cTn-I ELISA Kit was designed for the quantitative measurement of Dog cTn-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.