Human mannan-binding lectin serine peptidase 2 (MASP2)ELISA kit

Code CSB-E17966h
Size 96T,5×96T,10×96T
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Product Details

Target Name
mannan-binding lectin serine peptidase 2
Alternative Names
MASP2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, cerebrospinal fluid (CSF)
Detection Range
23.44 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 101  
Range % 91-111  
1:400 Average % 97  
Range % 91-108  
1:800 Average % 99  
Range % 92-106  
1:1600 Average % 95  
Range % 86-98  
Recovery
The recovery of human MASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-99  
EDTA plasma (n=4) 96 90-112  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.540 2.539 2.540 2.395  
750 2.022 2.027 2.025 1.880  
375 1.154 1.190 1.172 1.027  
187.5 0.702 0.760 0.731 0.586  
93.75 0.380 0.391 0.386 0.241  
46.88 0.280 0.300 0.290 0.145  
23.44 0.175 0.161 0.168 0.023  
0 0.140 0.150 0.145    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The human mannan-binding lectin serine peptidase 2 (MASP2) Elisa kit is suitable for the quantitative measurement of human MASP2 in serum, plasma, cell culture supernates, or cerebrospinal fluid (CSF). This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MASP2 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

MASP2 is a key enzyme for the activation of the lectin pathway of the complement system. MASP-2 is the exclusive MASP with the ability to cleave both C2 and C4 to form the LP C3 convertase C4bC2a and the C5 convertase C4bC2a(C3b)n. Deficiency in MASP2 is considered a primary immunodeficiency related to pyogenic bacterial infections, inflammatory lung disease, and autoimmunity. The level of MASP2 in plasma plays a dual role in the infection and progression of infectious diseases. The deficiency of MASP2 in plasma has been associated with susceptibility to HIV. On the contrary, the overproduction of MASP2 may increase the inflammatory effect.

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Target Background

Function
(From Uniprot)
Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
Gene References into Functions
  1. High MAp19 concentration is associated with microalbuminuria in newly diagnosed type 1 diabetes. PMID: 28303635
  2. The complement lectin pathway serine proteases, MASP-1 and MASP-2, can be associated with ischaemic stroke development risk and may participate in pathological events leading to post-ischaemic brain damage. Moreover rs3203210 and rs147270785 single nucleotide polymorphisms in the MASP1 and MASP2 genes, respectively, are strongly associated with ischaemic stroke. PMID: 28720568
  3. The results of this study show that MASP-2 contributes to severity and outcome in pneumococcal meningitis. In patients with pneumococcal meningitis, MASP-2 concentration was elevated in the CSF and high levels were associated with poor functional outcome. PMID: 28086930
  4. Genetically determined MASP-2 levels seem to have a two-edge effect in HIV and probably HCV/HBV coinfection, whereas low levels increase the susceptibility to infection, but on the other side protects against AIDS. PMID: 27588826
  5. MASP-2 was not associated with adverse cardiovascular outcomes in diabetics. PMID: 27055907
  6. This is the first comprehensive meta-analysis indicates that the MASP-2 functional gene (rs72550870) polymorphism is not associated with the infection diseases, and the key functional gene polymorphism of rs72550870 did not increase susceptibility to the infection diseases. PMID: 27725284
  7. This study suggests that hMASP-2 can induce a protective efficacy in BCG-infected rabbit skin models, which affects both the progress of lesions and the survival of the mycobacteria within them. PMID: 27585546
  8. genotype not associated with acute cellular rejection after kidney transplantation PMID: 26924055
  9. The ficolin-3/MASP-2 complex was significantly lower in the cardiac syndrome X patients compared to controls. PMID: 27312152
  10. MASP-1 and MASP-2 are activated during blood clotting. This activation is triggered by activated platelets and by the generation of fibrin during thrombotic reactions in vitro and in vivo, and may represent a novel activation/amplification mechanism in thromboinflammation. PMID: 26614707
  11. MBL2 and MASP2 deficiencies protect against the development of systemic inflammatory response after pediatric cardiac surgery. PMID: 26382056
  12. Data show that the vector expressing mannose-binding lectin associated protein 19 (MAp19) has been prepared successfully, and can express the target protein (MAp19) in the eukaryotic cells (HeLa cells). PMID: 26728378
  13. polymorphism of MASP-2 (rs6695096) gene was associated with susceptibility to tuberculosis. PMID: 25312983
  14. Polymorphisms of mannose-binding lectin (rs7096206) and MASP-2 (rs2273346 and rs6695096) were associated with the susceptibility of tuberculosis, and there were gene-gene interactions among them. PMID: 25887173
  15. Polymorphisms in MASP1 and MASP2 genes are associated with the susceptibility or protection to infectious diseases. (Review) PMID: 25862418
  16. MASP2 gene polymorphisms and protein levels seem to play an important role in the development of rheumatic fever and establishment of rheumatic heart disease. PMID: 25318078
  17. Because MASP-1 and MASP-2 have been shown to interact directly with blood coagulation, elevated levels of these proteins may play a role in the enhanced thrombotic environment and consequent vascular complications in diabetes. PMID: 25533914
  18. TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2. PMID: 25359215
  19. expression of MBL and MASP-2 is altered in ovarian cancer, possibly indicating involvement of the lectin pathway of complement activation in the disease PMID: 25038892
  20. Both ficolin-3 and MASP-2 levels correlated inversely with the time from the onset of the attack of hereditary angioedema until blood sampling PMID: 25042985
  21. Study found the first evidence that MASP-2 deficiency might play an important role in the development of RA and articular symptoms among relatives of RA patients. PMID: 24632598
  22. Bipolar disorder is associated with the MBL2 LXPA haplotype and lower MASP-2 levels. PMID: 24856568
  23. Data indicate that MASP-1 and MASP-2 can readily form heterodimers after dissociation and re-association, however, in the presence of Ca(2+) exchange of subunits is slow between the homodimers. PMID: 24424083
  24. low MASP-2 levels increase the susceptibility to leprosy in general and to lepromatous leprosy in particular. MASP2 genotypes and MASP-2 levels might thus be of prognostic value for leprosy progression. PMID: 23935922
  25. The present study describes an association between a polymorphism in the MASP2 gene and susceptibility to HTLV-1 infection, and provides further evidence of an association between the MBL2 gene and HTLV-1 infection. PMID: 23861212
  26. MASP-2 levels in the peripheral circulation are significantly reduced in myocardial infarction (MI) patients compared with those of healthy individuals or of coronary artery disease (CAD) patients without acute MI. PMID: 22178059
  27. results show that SNPS in MASP2 are significantly more susceptible to infectious complications, SIRS and septic shock. PMID: 24227370
  28. Studies indicate that initiation of lectin compleme pathway leads to activation of the serine proteases MASP-1 and MASP-2 resulting in deposition of C4 on the activator and assembly of the C3 convertase. PMID: 23911397
  29. collectin-11 associates with all the known MBL-associated serine proteases (MASP-1, MASP-2 and MASP-3) as well as the lectin complement pathway regulator MAP-1. PMID: 23220946
  30. In this study, we demonstrate that, although MASPs do not directly form heterodimers, the addition of mannan-binding lectin or ficolins allows the formation of MASP-1-MASP-2 co-complexes. PMID: 23785123
  31. Data indicate that the median concentration of MASP-2/ficolin-3 complexes was 119.7 AU/ml (range: 2.9-615.5 AU/ml). PMID: 23142462
  32. MASP-2 is a key enzyme that cleaves C4 and C2 to assemble a C3 convertase--{review} PMID: 23402018
  33. A crucial role of MASP-1 is demonstrated in the activation of MASP-2, as well as of MASP-3, based on a patient harboring a nonsense mutation in the common part of the MASP1 gene. PMID: 22966085
  34. Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. PMID: 22511776
  35. MASP-1 activates MASP-2 and, moreover, inhibition of MASP-1 prevents autoactivation of MASP-2 PMID: 22691502
  36. A common MASP2 mutation (R439H, rs12085877), was protective against placental malaria and occurred in 33% of non-affected women. PMID: 22380611
  37. Data show no association of donor and recipient MBL2 and MASP2 genotype with allograft outcome, and analysis of SNPs and haplotypes in the FCN2 gene of the donor and recipient did not reveal an association with transplant outcome. PMID: 22173059
  38. Interactions required for the cleavage of C4 by MASP-2 are likely to be facilitated by the initial binding of C4 to an exosite on the protease. PMID: 22071314
  39. In patients with haematological malignancy undergoing chemotherapy, those with low M-ficolin levels were more likely to develop severe infections. PMID: 22236007
  40. It is postulated that the elevation of concentration of the two components of the leptin pathway, L-ficolin and MASP-2, may compensate for the insufficient activity of the LP in mannose-binding lectin deficiency. PMID: 21974696
  41. Data show that both MAp19 and MASP-2 were mainly expressed in hepatocytes. PMID: 21871896
  42. MASP2 gene polymorphism is associated with susceptibility to hepatitis C virus infection. PMID: 21843573
  43. Results suggest that MBL and MASP-2 play only a minor role in the inflammatory response in acute pancreatitis. PMID: 21926545
  44. MASP2*CD genotypes, most of them generating low MASP-2 levels, are associated with high risk of chagasic cardiomyopathy PMID: 21489885
  45. binding of Mannan Binding Lectin to hepatitis C virus glycoproteins was able to activate the complement system via MBL-associated serine protease 2 PMID: 21203938
  46. In contrast to what has been demonstrated for serum levels of mannan-binding lectin (MBL) and MBL-associated serine protease 2, the genotypes do not predict disease course of the colorectal cancer patients PMID: 21198752
  47. Donor and recipient gene polymorphisms in the lectin complement pathway (MBL2, FCN2, MASP2) are major determinants of the risk of clinically significant bacterial infection and mortality after orthotopic liver transplantation. PMID: 20593422
  48. MASP-1 has a crucial role in the initiation steps of lectin pathway activation most probably by activating MASP-2 PMID: 20817870
  49. The MASP2-mediated release of FPA and FPB may play a role in early immune activation. Additionally, MASP-catalysed deposition and polymerization of fibrin on the surface of micro-organisms may be protective by limiting the dissemination of infection. PMID: 20002787
  50. the outcome of intensive care unit (ICU) patients with systemic inflammatory response syndrome (SIRS) regarding the existence of functionally relevant MBL2 and MASP2 gene polymorphisms was studied PMID: 20042521

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Involvement in disease
MASP2 deficiency (MASPD)
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Plasma.
Database Links

HGNC: 6902

OMIM: 605102

KEGG: hsa:10747

STRING: 9606.ENSP00000383690

UniGene: Hs.655645

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