Alternative Names
Blast 2 ELISA Kit; BLAST-2 ELISA Kit; Blast2 ELISA Kit; C type lectin domain family 4 member J ELISA Kit; C-type lectin domain family 4 ELISA Kit; C-type lectin domain family 4 member J ELISA Kit; C-type lectin domain family 4; member J ELISA Kit; CD 23 ELISA Kit; CD 23A ELISA Kit; CD23 ELISA Kit; CD23 antigen ELISA Kit; CD23A ELISA Kit; CLEC 4J ELISA Kit; CLEC4J ELISA Kit; Fc epsilon receptor II ELISA Kit; Fc epsilon RII ELISA Kit; Fc fragment of IgE ELISA Kit; Fc fragment of IgE low affinity II receptor for ELISA Kit; Fc fragment of IgE receptor II ELISA Kit; Fc fragment of IgE; low affinity II; receptor for (CD23) ELISA Kit; Fc of IgE ELISA Kit; Fc of IgE; low affinity II; receptor for (CD23) ELISA Kit; Fc receptor IgE low affinity II alpha polypeptide ELISA Kit; Fc receptor; IgE; low affinity II; alpha polypeptide; isoform CRA_a ELISA Kit; Fc-epsilon-RII ELISA Kit; FCE 2 ELISA Kit; FCE2 ELISA Kit; FCER 2 ELISA Kit; Fcer2 ELISA Kit; FCER2_HUMAN ELISA Kit; FCER2A ELISA Kit; FceRII ELISA Kit; IgE binding factor ELISA Kit; IgE receptor lymphocyte ELISA Kit; IgE-binding factor ELISA Kit; IGEBF ELISA Kit; Immunoglobulin E binding factor ELISA Kit; Immunoglobulin E receptor ELISA Kit; Immunoglobulin E receptor; low affinity II ELISA Kit; Immunoglobulin E-binding factor ELISA Kit; Immunoglobulin epsilon chain ELISA Kit; LEUKOCYTE ANTIGEN CD23 ELISA Kit; Low Affinity IgE Receptor ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor membrane bound form ELISA Kit; Low affinity immunoglobulin epsilon Fc receptor soluble form ELISA Kit; Ly-42 ELISA Kit; Ly42 ELISA Kit; Lymphocyte antigen CD23 ELISA Kit; Lymphocyte IgE receptor ELISA Kit; MGC93219 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sCD23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:1 |
Average % |
95 |
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Range % |
90-100 |
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1:2 |
Average % |
88 |
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Range % |
84-92 |
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1:4 |
Average % |
97 |
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Range % |
92-101 |
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1:8 |
Average % |
85 |
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Range % |
80-90 |
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Recovery
The recovery of human sCD23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
104 |
100-110 |
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EDTA plasma (n=4) |
96 |
91-100 |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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5000 |
2.651 |
2.495 |
2.573 |
2.418 |
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2500 |
1.903 |
1.827 |
1.865 |
1.710 |
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1250 |
1.390 |
1.306 |
1.348 |
1.193 |
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625 |
0.812 |
0.853 |
0.833 |
0.678 |
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312 |
0.549 |
0.529 |
0.539 |
0.384 |
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156 |
0.338 |
0.341 |
0.340 |
0.185 |
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78 |
0.264 |
0.260 |
0.262 |
0.107 |
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0 |
0.152 |
0.158 |
0.155 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx