Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

Code CSB-E09543m
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
anti-thyroid-globulin antibody,TGAB
Abbreviation
TG Ab
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
1.25 U/mL-20 U/mL
Sensitivity
1.25 U/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-101
1:2 Average % 89
Range % 85-98
1:4 Average % 92
Range % 84-100
1:8 Average % 95
Range % 90-100
Recovery
The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-103
EDTA plasma (n=4) 95 90-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/ml OD1 OD2 Average  
20 0.146 0.151 0.149  
10 0.278 0.281 0.280  
5 0.457 0.463 0.460  
2.5 0.854 0.870 0.862  
1.25 1.865 1.828 1.847  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse TG Ab ELISA Kit was designed for the quantitative measurement of Mouse TG Ab protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.25 U/mL-20 U/mL and the sensitivity is 1.25 U/mL.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Mouse

Sample dilution: 1: 10

Review: I used CSB-E09543m to detect autoimmune thyroiditis rats, and the dilution ratio was 1: 10. The experimental results were also accurate and in line with the trend. We expect to produce the TGAB box for rats, and there is a great demand in our laboratory.

By Anonymous

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