Code | CSB-AP002891HU |
MSDS | |
Size | $142 |
Order now | |
Image | |
Have Questions? | Leave a Message or Start an on-line Chat |
The production of recombinant human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF) involves inserting the gene encoding the MANF protein (25-182aa) into a vector and transferring it into E.coli cells. The E.coli cells are grown in bioreactors, where they express the recombinant MANF protein. The MANF protein is purified from the cell lysate using chromatographic techniques, yielding a high-purity product that can be used in research. Its activity has been validated in a cell proliferation assay using rat C6 cells, with the ED50 less than 20 μg/ml, corresponding to a specific activity of >50 IU/mg. It contains less than 1.0 EU/μg endotoxin as determined by the LAL method. Its purity is over 95% as measured by SDS-PAGE.
MANF a member of the family of evolutionarily conserved neurotrophic factors, is known for its neuroprotective and neurorestorative properties [1][2]. It can be induced by endoplasmic reticulum (ER) stress [3]. It is localized in the ER and promotes cell survival [4]. MANF is expressed in immune cells and has an autocrine role in immune modulation, promoting anti-inflammatory activation in both flies and mice [5]. MANF represents a unique class of neurotrophic factors, distinct in structure and function from classical neurotrophic factors [6]. Recent research underscores the crucial role of MANF in maintaining ER homeostasis [7]. It reduces cell apoptosis by upregulating GRP78, promoting the survival of dopaminergic neurons [8]. Furthermore, MANF protects dopaminergic neurons and is involved in the ER unfolded protein response [9]. When injected into the brain, recombinant mammalian MANF has been shown to protect and repair dopaminergic neurons in toxin-induced rodent models of Parkinson’s disease (PD) in vivo [10].
References:
[1] K. Mätlik, J. Anttila, K. Tseng, O. Smolander, E. Pakarinen, L. Lehtonenet al., Poststroke delivery of manf promotes functional recovery in rats, Science Advances, vol. 4, no. 5, 2018. https://doi.org/10.1126/sciadv.aap8957
[2] L. Belayev, S. Hong, R. Freitas, H. Menghani, S. Marcell, L. Khoutorovaet al., Dha modulates manf and trem2 abundance, enhances neurogenesis, reduces infarct size, and improves neurological function after experimental ischemic stroke, CNS Neuroscience & Therapeutics, vol. 26, no. 11, p. 1155-1167, 2020. https://doi.org/10.1111/cns.13444
[3] X. Wang, M. Song, S. Bi, Y. Shen, & Y. Yan, Mri dynamically evaluates the therapeutic effect of recombinant human manf on ischemia/reperfusion injury in rats, International Journal of Molecular Sciences, vol. 17, no. 9, p. 1476, 2016. https://doi.org/10.3390/ijms17091476
[4] E. Pakarinen, T. Danilova, V. Voikar, P. Chmielarz, P. Piepponen, M. Airavaaraet al., Manf ablation causes prolonged activation of the upr without neurodegeneration in the mouse midbrain dopamine system, Eneuro, vol. 7, no. 1, p. ENEURO.0477-19.2019, 2020. https://doi.org/10.1523/eneuro.0477-19.2019
[5] P. Sousa‐Victor, J. Neves, W. Cedron-Craft, P. Ventura, C. Liao, R. Rileyet al., Manf regulates metabolic and immune homeostasis in ageing and protects against liver damage, Nature Metabolism, vol. 1, no. 2, p. 276-290, 2019. https://doi.org/10.1038/s42255-018-0023-6
[6] J. Guo, Y. Cui, Q. Liu, Y. Yang, Y. Li, L. Wenget al., Piperine ameliorates sca17 neuropathology by reducing er stress, Molecular Neurodegeneration, vol. 13, no. 1, 2018. https://doi.org/10.1186/s13024-018-0236-x
[7] H. Montaser, K. Patel, D. Balboa, H. Ibrahim, V. Lithovius, A. Näätänenet al., Loss of manf causes childhood-onset syndromic diabetes due to increased endoplasmic reticulum stress, Diabetes, vol. 70, no. 4, p. 1006-1018, 2021. https://doi.org/10.2337/db20-1174
[8] J. Huang, C. Chen, H. Gu, C. Li, X. Fu, M. Jianget al., Mesencephalic astrocyte‐derived neurotrophic factor reduces cell apoptosis via upregulating grp78 in sh‐sy5y cells, Cell Biology International, vol. 40, no. 7, p. 803-811, 2016. https://doi.org/10.1002/cbin.10621
[9] C. Richman, S. Rashid, S. Prashar, R. Mishra, S. Pr, & B. Gupta, C. elegans manf homolog is necessary for the protection of dopaminergic neurons and er unfolded protein response, Frontiers in Neuroscience, vol. 12, 2018. https://doi.org/10.3389/fnins.2018.00544
[10] R. Lindström, P. Lindholm, J. Kallijärvi, M. Palgi, M. Saarma, & T. Heino, Exploring the conserved role of manf in the unfolded protein response in drosophila melanogaster, Plos One, vol. 11, no. 3, p. e0151550, 2016. https://doi.org/10.1371/journal.pone.0151550
There are currently no reviews for this product.