Recombinant Arabidopsis thaliana APO protein 1, chloroplastic (APO1)

Code CSB-YP893147DOA
MSDS
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Source Yeast
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Code CSB-EP893147DOA
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Source E.coli
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Code CSB-EP893147DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893147DOA
MSDS
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Source Baculovirus
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Code CSB-MP893147DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
APO1
Uniprot No.
Alternative Names
APO1; At1g64810; F13O11.11APO protein 1; chloroplastic; Accumulation of photosystem I protein 1; Protein ACCUMULATION OF PHOTOSYSTEM ONE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
48-436
Target Protein Sequence
LGT VFCFNQKHRE QTSFKKRYVS TQNVDLPPIL PKNKKKPYPI PFKQIQEEAR KDKKLAQMGI EKQLDPPKNG LLVPNLVPVA DQVIDNWKLL IKGLAQLLHV VPVFACSECG AVHVANVGHN IRDCNGPTNS QRRGSHSWVK GTINDVLIPV ESYHMYDPFG RRIKHETRFE YERIPALVEL CIQAGVEIPE YPCRRRTQPI RMMGKRVIDR GGYHKEPEKP QTSSSLSSPL AELDTLGVFE RYPPPTPEDI PKIAQETMDA YEKVRLGVTK LMRKFTVKAC GYCSEVHVGP WGHSVKLCGE FKHQWRDGKH GWQDALVDEV FPPNYVWHVR DLKGNPLTGN LRRFYGKAPA LVEICMHSGA RVPQRYKAMM RLDIIVPDSQ EADMVA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the stable assembly of several 4Fe-4S cluster-containing complexes of chloroplasts. May participate in 4Fe-4S cofactor incorporation into psaA and/or psaB during translation.
Gene References into Functions
  1. APO1 is a zinc binding protein and harbors motifs that resemble zinc fingers. It promotes the splicing of several chloroplast group II introns. PMID: 21421812
  2. APO1 is involved in the stable assembly of several [4Fe-4S] cluster-containing complexes of chloroplasts and interferes with translational events probably in association with plastid nucleoids. [PO1] PMID: 15494558
Subcellular Location
Plastid, chloroplast.
Protein Families
APO family
Tissue Specificity
Expressed at low level. Expressed at higher level in leaves. Expressed at lower level in roots, stems, siliques and flowers.
Database Links

KEGG: ath:AT1G64810

STRING: 3702.AT1G64810.2

UniGene: At.28084

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