Recombinant Arabidopsis thaliana Agamous-like MADS-box protein AGL8 (AGL8)

Code CSB-YP654335DOA
MSDS
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Source Yeast
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Code CSB-EP654335DOA
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Source E.coli
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Code CSB-EP654335DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP654335DOA
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Source Baculovirus
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Code CSB-MP654335DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGL8
Uniprot No.
Alternative Names
AGL8; FUL; At5g60910; MSL3.3Agamous-like MADS-box protein AGL8; Floral homeotic protein AGL8; Transcription factor FRUITFULL
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-242
Target Protein Sequence
MGRGRVQLKR IENKINRQVT FSKRRSGLLK KAHEISVLCD AEVALIVFSS KGKLFEYSTD SCMERILERY DRYLYSDKQL VGRDVSQSEN WVLEHAKLKA RVEVLEKNKR NFMGEDLDSL SLKELQSLEH QLDAAIKSIR SRKNQAMFES ISALQKKDKA LQDHNNSLLK KIKEREKKTG QQEGQLVQCS NSSSVLLPQY CVTSSRDGFV ERVGGENGGA SSLTEPNSLL PAWMLRPTTT NE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1 and CAULIFLOWER. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Seems to be partially redundant to the function of APETALA1 and CAULIFLOWER in the up-regulation of LEAFY. Is also required for normal pattern of cell division, expansion and differentiation during morphogenesis of the silique. Probably not required for fruit elongation but instead is required to prevent ectopic activity of IND. Represses SAUR10 expression in stems and inflorescence branches.
Gene References into Functions
  1. SAUR10 is repressed by FUL in stems and inflorescence branches. SAUR10 is specifically expressed at the abaxial side of these branches and this localized activity is influenced by hormones, light conditions and by FUL, which has an effect on branch angle. PMID: 28586421
  2. Two cis-elements were involved in the repression of FUL expression. The first intron of FUL was shown to participate in the development of carpel and stamen as an enhancer. PMID: 21284215
Subcellular Location
Nucleus.
Tissue Specificity
Vascular tissue of cauline leaves, floral shoot apex and valves of carpels and fruits.
Database Links

KEGG: ath:AT5G60910

STRING: 3702.AT5G60910.1

UniGene: At.5426

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