Recombinant Arabidopsis thaliana Bifunctional dihydrocamalexate synthase/camalexin synthase (CYP71B15), partial

Code CSB-YP868053DOA
MSDS
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Source Yeast
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Code CSB-EP868053DOA
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Source E.coli
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Code CSB-EP868053DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868053DOA
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Source Baculovirus
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Code CSB-MP868053DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP71B15
Uniprot No.
Alternative Names
CYP71B15; PAD3; At3g26830; MDJ14.15; Bifunctional dihydrocamalexate synthase/camalexin synthase; EC 1.14.19.52; Cytochrome P450 71B15; Dihydrocamalexate:NADP(+) oxidoreductase; decarboxylating); Protein PHYTOALEXIN DEFICIENT 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.
Gene References into Functions
  1. PAD3 is also required for induced resistance to green peach aphid, independently of BAK1 and reactive oxygen species production. PMID: 24586042
  2. PAD3 catalyzes the conversion of dihydrocamalexic acid to camalexin, the final step in camalexin biosynthesis. PMID: 16766671
  3. PHYTOALEXIN DEFICIENT3 (PAD3) is strongly up-regulated by both oligogalacturonides and Botrytis infection independently of salicylic acid, jasmonate, and ethylene. PMID: 17384165
  4. Indole-3-acetonitrile is derivatized to the intermediate Cys(IAN), which serves as substrate of the multifunctional cytochrome P450 enzyme CYP71B15. PMID: 19567706
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Database Links

KEGG: ath:AT3G26830

STRING: 3702.AT3G26830.1

UniGene: At.6033

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