Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 3 (CIPK3)

Code CSB-YP648860DOA
MSDS
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Source Yeast
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Code CSB-EP648860DOA
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Source E.coli
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Code CSB-EP648860DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP648860DOA
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Source Baculovirus
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Code CSB-MP648860DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CIPK3
Uniprot No.
Alternative Names
CIPK3; PKS12; SnRK3.17; At2g26980; T20P8.3CBL-interacting serine/threonine-protein kinase 3; EC 2.7.11.1; SNF1-related kinase 3.17; SOS2-like protein kinase PKS12
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-441
Target Protein Sequence
MNRRQQVKRR VGKYEVGRTI GEGTFAKVKF ARNSETGEPV ALKILDKEKV LKHKMAEQIR REIATMKLIK HPNVVQLYEV MASKTKIFII LEYVTGGELF DKIVNDGRMK EDEARRYFQQ LIHAVDYCHS RGVYHRDLKP ENLLLDSYGN LKISDFGLSA LSQQVRDDGL LHTSCGTPNY VAPEVLNDRG YDGATADMWS CGVVLYVLLA GYLPFDDSNL MNLYKKISSG EFNCPPWLSL GAMKLITRIL DPNPMTRVTP QEVFEDEWFK KDYKPPVFEE RDDSNMDDID AVFKDSEEHL VTEKREEQPA AINAFEIISM SRGLNLENLF DPEQEFKRET RITLRGGANE IIEKIEEAAK PLGFDVQKKN YKMRLENVKA GRKGNLNVAT EIFQVAPSLH MVQVSKSKGD TLEFHKFYKK LSNSLEQVVW TNNEVKKETA K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the resistance to some abiotic stresses (e.g. high salt, hyperosmotic stress) in young seedlings, by regulating the expression of several stress-inducible genes (cold- and salt-induced genes but not drought-responsive genes). Required for the ABA response during germination. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. The CBL9/CIPK3 complex acts in the regulation of abscisic acid response in seed germination.
Gene References into Functions
  1. The CBL9-CIPK3-ABR1 pathway functions to regulate seed germination and ABA dependent physiological processes in Arabidopsis. PMID: 27964784
  2. CBL9 and CIPK3 act together in the same pathway for regulating abscisic acid responses in Arabidopsis. PMID: 19825536
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Mostly expressed in germinating seeds and young seedlings. Detected at low levels in roots, stems, leaves and flowers.
Database Links

KEGG: ath:AT2G26980

STRING: 3702.AT2G26980.4

UniGene: At.13161

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