Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 6 (CIPK6)

Code CSB-YP528972DOA
MSDS
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Source Yeast
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Code CSB-EP528972DOA
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Source E.coli
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Code CSB-EP528972DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528972DOA
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Source Baculovirus
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Code CSB-MP528972DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CIPK6
Uniprot No.
Alternative Names
CIPK6; PKS4; SIP3; SnRK3.14; At4g30960; F6I18.130CBL-interacting serine/threonine-protein kinase 6; EC 2.7.11.1; SNF1-related kinase 3.14; SOS2-like protein kinase PKS4; SOS3-interacting protein 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-441
Target Protein Sequence
MVGAKPVENG SDGGSSTGLL HGRYELGRLL GHGTFAKVYH ARNIQTGKSV AMKVVGKEKV VKVGMVDQIK REISVMRMVK HPNIVELHEV MASKSKIYFA MELVRGGELF AKVAKGRLRE DVARVYFQQL ISAVDFCHSR GVYHRDLKPE NLLLDEEGNL KVTDFGLSAF TEHLKQDGLL HTTCGTPAYV APEVILKKGY DGAKADLWSC GVILFVLLAG YLPFQDDNLV NMYRKIYRGD FKCPGWLSSD ARRLVTKLLD PNPNTRITIE KVMDSPWFKK QATRSRNEPV AATITTTEED VDFLVHKSKE ETETLNAFHI IALSEGFDLS PLFEEKKKEE KREMRFATSR PASSVISSLE EAARVGNKFD VRKSESRVRI EGKQNGRKGK LAVEAEIFAV APSFVVVEVK KDHGDTLEYN NFCSTALRPA LKDIFWTSTP A
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Downstream of CBL1, CBL2, CBL3 and CBL9, regulates by phosphorylation the K(+) conductance and uptake of AKT1. Binds to CBL4 to modulate AKT2 activity by promoting a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.
Gene References into Functions
  1. A calcium-regulated protein kinase Calcineurin B-like protein (CBL)-interacting protein kinase 6 (CIPK6) functions as a negative regulator of immunity against the bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. PMID: 28541442
  2. Arabidopsis CBL-interacting protein kinase (CIPK6) is involved in plant response to salt/osmotic stress and abscisic acid. PMID: 23649767
  3. Transgenic seedlings expressing CIPK3 displayed increased root length, overall root branching and hypersensitivity to auxin. Mutation caused defects in seedling development and auxin transport. PMID: 19187042
Subcellular Location
Endoplasmic reticulum. Note=Targeted to the cell membrane when interacting with CBL4 and ATK2.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Expressed in roots and shoots.
Database Links

KEGG: ath:AT4G30960

STRING: 3702.AT4G30960.1

UniGene: At.2154

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