Recombinant Arabidopsis thaliana DNA repair protein XRCC4 (XRCC4)

Code CSB-YP715001DOA
MSDS
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Source Yeast
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Code CSB-EP715001DOA
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Source E.coli
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Code CSB-EP715001DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP715001DOA
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Source Baculovirus
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Code CSB-MP715001DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XRCC4
Uniprot No.
Alternative Names
XRCC4; At3g23100; MXC7.14; DNA repair protein XRCC4
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-264
Target Protein Sequence
MIGVDSKSSS TTFIETMVES EKTKHTCLRL EISGADPIFV KGTWHNSRFD ISVTDGSSSW ICNATEEEVA ERAAQWDQPV SEYLKLAEQY LGFQQPNSVY SFSDALEGSK RLSWTFEKEG TKLEWRWKCK PSDDSKKITV GILDFLMEAN IRLSEEVVNK TRSFEKMRSE AERCLAQGEK LCDEKTEFES ATYAKFLSVL NAKKAKLRAL RDKEDSVRVV EEEESTDKAE SFESGRSDDE KSEEEASKKA TSSKARGGKR AARS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. May bind to DNA. The LIG4-XRCC4 complex is probably responsible for the NHEJ ligation step, and XRCC4 may enhance the joining activity of LIG4.
Gene References into Functions
  1. XRCC4 has a negative role in Agrobacterium T-DNA integration. PMID: 23064322
Subcellular Location
Nucleus.
Protein Families
XRCC4 family
Database Links

KEGG: ath:AT3G23100

STRING: 3702.AT3G23100.1

UniGene: At.36458

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