Recombinant Arabidopsis thaliana Disease resistance protein RPM1 (RPM1), partial

Code CSB-YP657193DOA
MSDS
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Source Yeast
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Code CSB-EP657193DOA
MSDS
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Source E.coli
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Code CSB-EP657193DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657193DOA
MSDS
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Source Baculovirus
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Code CSB-MP657193DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPM1
Uniprot No.
Alternative Names
RPM1; RPS3; At3g07040; F17A9.20; Disease resistance protein RPM1; Resistance to Pseudomonas syringae protein 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Disease resistance (R) protein that specifically recognizes the AvrRpm1 type III effector avirulence protein from Pseudomonas syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Acts via its interaction with RIN4, and probably triggers the plant resistance when RIN4 is phosphorylated by AvrRpm1. It is then degraded at the onset of the hypersensitive response.
Gene References into Functions
  1. RPM1, RPS2 and RPS5 are R proteins. Here, we provide evidence that FLS2 is physically associated with all three R proteins. PMID: 21726371
  2. RPM1 is activated at, and functions on, the plasma membrane. PMID: 21490299
  3. Threonine phosphorylation of Arabidopsis RIN4 by Pseudomonas AvrRpm1 and AvrB activates Arabidopsis immune receptor RPM1. PMID: 21320695
  4. Data show that a PP2C-type phosphatase (renamed PIA1) was identified that accumulates following infection by Pseudomonas syringae expressing AvrRpm1 and subsequent activation of the corresponding plant NB-LRR disease resistance protein RPM1. PMID: 19843314
  5. Two closely related RPM1-interacting proteins, RIN2 and RIN3, are characterized in A. thaliana. PMID: 16212605
  6. AvrB is activated inside the host cell by nucleotide binding and subsequent phosphorylation and interacts with RIN4. Aactivated AvrB, bound to RIN4, is indirectly recognized by RPM1 to initiate plant immune system function. PMID: 17397263

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Subcellular Location
Endomembrane system; Peripheral membrane protein.
Protein Families
Disease resistance NB-LRR family
Database Links

KEGG: ath:AT3G07040

STRING: 3702.AT3G07040.1

UniGene: At.40415

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