Recombinant Arabidopsis thaliana Eukaryotic translation initiation factor 3 subunit F (TIF3F1)

Code CSB-YP007535DOA
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Source Yeast
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Code CSB-EP007535DOA
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Source E.coli
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Code CSB-EP007535DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007535DOA
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Source Baculovirus
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Code CSB-MP007535DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIF3F1
Uniprot No.
Alternative Names
TIF3F1; At2g39990; T29M21.15Eukaryotic translation initiation factor 3 subunit F; eIF3f; eIF-3-epsilon; eIF3 p32 subunit
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-293
Target Protein Sequence
MAATSEHTIL QFVSPSSTAS ATTSVLTARI HPLVIFNVCD CFVRRPDSAE RVIGTLLGSI LPDGTVDIRN SYAVPHNESS DQVAVDIDYH HNMLASHLKV NSKETIVGWY STGAGVNGGS SLIHDFYARE VPNPIHLTVD TGFTNGEGTI KAFVSSNLSL GDRQLVAHFQ EIPVDLRMVD AERVGFDVLK ATSVDKLPND LEGMELTMER LLTLINDVYK YVDSVVGGQI APDNNIGRFI ADAVASLPKL PPQVFDNLVN DSLQDQLLLL YLSSITRTQL SLAEKLNTAA QML
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Involved in cell growth and differentiation, especially during embryogenesis and male gametophyte germination. Regulates sensitivity to sugars (e.g. sucrose).
Gene References into Functions
  1. AteIF3f might play important roles in Arabidopsis cell growth and differentiation in combination with eIF3e and eIF3h. [eIF3f] PMID: 20444226
Subcellular Location
Cytoplasm.
Protein Families
EIF-3 subunit F family
Tissue Specificity
Expressed in inflorescences, leaves, stems, siliques, roots and seedlings. Accumulates at highly levels in pollen grains, developing embryos and root tips.
Database Links

KEGG: ath:AT2G39990

STRING: 3702.AT2G39990.1

UniGene: At.10968

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