Recombinant Arabidopsis thaliana Homeobox-leucine zipper protein ATHB-14 (ATHB-14), partial

Code CSB-YP522349DOA
MSDS
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Source Yeast
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Code CSB-EP522349DOA
MSDS
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Source E.coli
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Code CSB-EP522349DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522349DOA
MSDS
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Source Baculovirus
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Code CSB-MP522349DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATHB-14
Uniprot No.
Alternative Names
ATHB-14; PHB; At2g34710; T29F13.8Homeobox-leucine zipper protein ATHB-14; HD-ZIP protein ATHB-14; Homeodomain transcription factor ATHB-14; Protein PHABULOSA
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable transcription factor involved in the determination of adaxial-abaxial polarity in ovule primordium. Specifies adaxial leaf fates.
Gene References into Functions
  1. In Planta Single-Molecule Pull-Down Reveals Tetrameric Stoichiometry of HD-ZIPIII:LITTLE ZIPPER Complexes. PMID: 27385814
  2. PHABULOSA, directly binds the promoter of both MONOPTEROS (MP)/AUXIN RESPONSE FACTOR5, a key factor in vascular formation. PMID: 26637548
  3. our study suggests that PHB can dynamically regulate TA cell activities in a QC-independent manner, and that the SHR-PHB pathway enables a robust root growth system by coordinating the stem cell niche and TA domain. PMID: 25730098
  4. findings show that the HD-ZIPIII transcription factor PHABULOSA directly activates the cytokinin biosynthesis gene ISOPENTENYL TRANSFERASE 7, thus promoting cell differentiation and regulating root length PMID: 22902752
  5. Pairing to the 5' portion of the small silencing RNA of PHAVOLUTA appears crucial regardless of the mode of post-transcriptional repression PMID: 15282547
  6. Class III HD-Zip genes required for root development [PHABULOSA (PHB)] PMID: 15286295

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Subcellular Location
Nucleus.
Protein Families
HD-ZIP homeobox family, Class III subfamily
Tissue Specificity
Expressed in the center of the meristem and on the adaxial side of the leaves.
Database Links

KEGG: ath:AT2G34710

STRING: 3702.AT2G34710.1

UniGene: At.37771

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