Recombinant Arabidopsis thaliana Peroxisomal membrane protein 13 (PEX13)

Code CSB-YP865956DOA
MSDS
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Source Yeast
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Code CSB-EP865956DOA
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Source E.coli
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Code CSB-EP865956DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865956DOA
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Source Baculovirus
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Code CSB-MP865956DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEX13
Uniprot No.
Alternative Names
PEX13; AMC; APM2; At3g07560; F21O3.27; Peroxisomal membrane protein 13; ABSTINENCE BY MUTUAL CONSENT; Peroxin-13; AtPEX13; Peroxisome biogenesis protein 13; Pex13p; Protein ABERRANT PEROXISOME MORPHOLOGY 2; Protein AMC
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-304
Target Protein Sequence
MASQPAGGSP PKPWEKEGNT SGPNPFRPPS NTSTAGSVEA SGTANPGEVV PPPVNRPNTA ANMNSLSRPV PARPWEQQNY GSTMGGGYGS NLGMTSGYGS GTYGSALGGY GSSYGGGMYG GSSMYRGGYG GGGLYGSSGM YGGGAMGGYG GTMGGYGMGM GTGMGMGMGM GMGGPYGSQD PNDPFNQPPS PPGFWISFLR VMQGAVNFFG RVAMLIDQNT QAFHMFMSAL LQLFDRGGML YGELARFVLR MLGVRTRPRK MQQPPQGPNG LPLPHQPHGN QNYLEGPKTA APGGGGGWDN VWGN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in PTS1- and PTS2-dependent protein import into peroxisomes. Required for PTS1-dependent protein import into pollen peroxisomes. Acts as a docking factor on peroxisomal membranes. Required for the proper targeting of PEX7 to the peroxisome. Required for the export/release of receptors on the peroxisome membrane. Essential for pollen-tube discharge that take place only in the presence of functional peroxisomes in either the male or the female gametophyte.
Gene References into Functions
  1. PEX13 has a role in plant peroxisome receptor docking and matrix protein import. PMID: 25008153
  2. pex13-1 allele had deleterious effects when combined with pex5-1 and pex14-2, which are defective in early-acting peroxins, as shown by reduced matrix protein import and enhanced physiological defects PMID: 20969679
  3. AMC is strongly and transiently expressed in both male and female gametophytes during fertilization and functions in gametophytes as a peroxin essential for protein import into peroxisomes. PMID: 18160292
  4. APM2/PEX13 and APM4/PEX12 have roles in both PTS1- and PTS2-dependent protein transport on the peroxisomal membrane. [APM2] [PEX13] PMID: 16813573
Subcellular Location
Peroxisome membrane; Peripheral membrane protein. Peroxisome.
Protein Families
Peroxin-13 family
Tissue Specificity
Highly expressed in pollen. Detected in shoots, roots, stems, leaves, inflorescences and emasculated postils. Strongly expressed in both male and female gametophytes during fertilization.
Database Links

KEGG: ath:AT3G07560

STRING: 3702.AT3G07560.1

UniGene: At.28242

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