Recombinant Arabidopsis thaliana Phospholipase D delta (PLDDELTA), partial

Code CSB-YP880190DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP880190DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP880190DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP880190DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP880190DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
PLDDELTA
Uniprot No.
Alternative Names
PLDDELTA; At4g35790; F4B14.60; Phospholipase D delta; AtPLDdelta; PLD delta; EC 3.1.4.4
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). May be involved in PA accumulation in the dehydration stress response and in the transduction of hormonal and environmental signals to the microtubules cytoskeleton. Prefers phosphatidylethanolamine to phosphatidylcholine as substrate. Involved in H(2)O(2) and abscisic acid (ABA)-induced stomatal closure. Involved in nitric oxide (NO) signaling during stomatal closure. Plays a positive role in ABA-promoted senescence. Involved in basal defense and nonhost resistance.
Gene References into Functions
  1. PLDdelta negatively regulates plant thermotolerance via destabilizing cortical microtubules. PMID: 28710795
  2. in wt plants PLDdelta disrupt membranes in severe drought stress and, in the absence of the protein (PLDdelta knock-out) might drought-prime the plants, making them more tolerant to severe drought stress. PMID: 26340512
  3. PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and PLDdelta is the main isoform participating in this process. PMID: 23979971
  4. PLDdelta-mediated hydrolysis of phosphatidylcholine to phosphatidic acid plays a positive role in abscisic acid-promoted senescence. PMID: 23762411
  5. Phospholipase Ddelta is downstream of nitric oxide and hydrogen peroxide in abscisic acid (ABA)-induced stomatal closure. PMID: 22932846
  6. Report show that the presence of PLDdelta correlates with reduced lipid hydrolysis during post-freezing recovery, suggesting a positive role for PLDdelta in freezing tolerance. PMID: 17962199

Show More

Hide All

Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
Phospholipase D family, C2-PLD subfamily
Tissue Specificity
Expressed in roots, leaves, stems, siliques and flowers. Strongly expressed in the vascular tissues of cotyledons and leaves under dehydration stress conditions. Expression is higher in old leaves than in young leaves. Expressed in leaves and guard cells.
Database Links

KEGG: ath:AT4G35790

STRING: 3702.AT4G35790.1

UniGene: At.22959

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1