Recombinant Arabidopsis thaliana Proline dehydrogenase 1, mitochondrial (POX1)

Code CSB-YP311282DOA
MSDS
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Source Yeast
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Code CSB-EP311282DOA
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Source E.coli
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Code CSB-EP311282DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP311282DOA
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Source Baculovirus
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Code CSB-MP311282DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POX1
Uniprot No.
Alternative Names
POX1; ERD5; PRO1; PRODH1; At3g30775; MIF6.16; MIF6.19Proline dehydrogenase 1; mitochondrial; EC 1.5.5.2; Osmotic stress-induced proline dehydrogenase; Proline oxidase; Protein EARLY RESPONSIVE TO DEHYDRATION 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
17-499
Target Protein Sequence
LPAF SPVGPPTVTA STAVVPEILS FGQQAPEPPL HHPKPTEQSH DGLDLSDQAR LFSSIPTSDL LRSTAVLHAA AIGPMVDLGT WVMSSKLMDA SVTRGMVLGL VKSTFYDHFC AGEDADAAAE RVRSVYEATG LKGMLVYGVE HADDAVSCDD NMQQFIRTIE AAKSLPTSHF SSVVVKITAI CPISLLKRVS DLLRWEYKSP NFKLSWKLKS FPVFSESSPL YHTNSEPEPL TAEEERELEA AHGRIQEICR KCQESNVPLL IDAEDTILQP AIDYMAYSSA IMFNADKDRP IVYNTIQAYL RDAGERLHLA VQNAEKENVP MGFKLVRGAY MSSEASLADS LGCKSPVHDT IQDTHSCYND CMTFLMEKAS NGSGFGVVLA THNADSGRLA SRKASDLGID KQNGKIEFAQ LYGMSDALSF GLKRAGFNVS KYMPFGPVAT AIPYLLRRAY ENRGMMATGA HDRQLMRMEL KRRLIAGIA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Converts proline to delta-1-pyrroline-5-carboxylate.
Gene References into Functions
  1. ProDH1 and ProDH2 are both necessary to achieve maximum resistance against Pseudomonas syringae pv. tomato AvrRpm1 and B. cinerea.[ProDH1] PMID: 27526663
  2. ARR18 interaction negatively interferes with the transcriptional activity of bZIP63 on the PDH1 promoter. [PDH1] PMID: 24948556
  3. ProDH potentiates the oxidative burst and cell death associated with the hypersensitive response. PMID: 21757996
  4. ProDH-silenced plants displayed reduced ROS and cell death levels as well as enhanced susceptibility in response to avirulent pathogens. PMID: 21311034
  5. bZIP11 target processes are identified using transiently increased nuclear bZIP11 levels and genome-wide expression analysis; the effect of bZIP11 on ASN1 and GBF6 are reported. PMID: 18088315

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Subcellular Location
Mitochondrion.
Protein Families
Proline oxidase family
Tissue Specificity
Ubiquitous. Highest expression in pollen grains, in the stigma and in developing embryos.
Database Links

KEGG: ath:AT3G30775

STRING: 3702.AT3G30775.1

UniGene: At.24174

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