Recombinant Arabidopsis thaliana Protein CUP-SHAPED COTYLEDON 1 (NAC054)

Code CSB-YP887837DOA
MSDS
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Source Yeast
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Code CSB-EP887837DOA
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Source E.coli
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Code CSB-EP887837DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887837DOA
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Source Baculovirus
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Code CSB-MP887837DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAC054
Uniprot No.
Alternative Names
NAC054; CUC1; NAC1; At3g15170; F4B12.8; Protein CUP-SHAPED COTYLEDON 1; NAC domain-containing protein 1; NAC domain-containing protein 54; ANAC054; NAC domain-containing protein CUC1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-310
Target Protein Sequence
MDVDVFNGWG RPRFEDESLM PPGFRFHPTD EELITYYLLK KVLDSNFSCA AISQVDLNKS EPWELPEKAK MGEKEWYFFT LRDRKYPTGL RTNRATEAGY WKATGKDREI KSSKTKSLLG MKKTLVFYKG RAPKGEKSCW VMHEYRLDGK FSYHYISSSA KDEWVLCKVC LKSGVVSRET NLISSSSSSA VTGEFSSAGS AIAPIINTFA TEHVSCFSNN SAAHTDASFH TFLPAPPPSL PPRQPRHVGD GVAFGQFLDL GSSGQIDFDA AAAAFFPNLP SLPPTVLPPP PSFAMYGGGS PAVSVWPFTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator of STM and KNAT6. Involved in molecular mechanisms regulating shoot apical meristem (SAM) formation during embryogenesis and organ separation. Required for the fusion of septa of gynoecia along the length of the ovaries. Activates the shoot formation in callus in a STM-dependent manner. Seems to act as an inhibitor of cell division.
Gene References into Functions
  1. cuc1 grf1/2/3, cuc2 grf1/2/3, and cuc3 grf1/2/3 quadruple mutants showed dramatic increases in cotyledon fusion as well as floral organ fusion. PMID: 25761011
  2. CUC1, CUC2, and AINTEGUMENTA have additive effects on ovule primordia formation. PMID: 23941199
  3. suppresses growth of sepal tissues from the boundary region PMID: 22507233
  4. SPT negatively regulates CUC1 and CUC2 expression in the apical part of the gynoecium. PMID: 22514090
  5. The results bring new insights into the mechanistic links between KNOXI and CUC transcription factors and contribute to the understanding of the regulatory network controlled by STM. PMID: 21685178
  6. CUC1 and CUC2 resulted from duplications of a unique ancestral gene and show different patterns of evolution. PMID: 21258003
  7. The molecular basis for the activity of the protein. PMID: 15500463
  8. These results suggest that ESR2 plays a role in shoot regeneration through transcriptional regulation of CUC1. PMID: 17056621
  9. Regulation of axillary meristem formation by miR164 is mediated through CUC1 and CUC2, which in turn regulate LAS. PMID: 18346190

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in inflorescence stems, rosette leaves, aerial parts of seedlings, flowers, floral buds and roots.
Database Links

KEGG: ath:AT3G15170

STRING: 3702.AT3G15170.1

UniGene: At.5743

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