Recombinant Arabidopsis thaliana Serine/threonine-protein kinase TOR (TOR), partial

Code CSB-YP861872DOA
MSDS
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Source Yeast
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Code CSB-EP861872DOA
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Source E.coli
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Code CSB-EP861872DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861872DOA
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Source Baculovirus
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Code CSB-MP861872DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TOR
Uniprot No.
Alternative Names
TOR; At1g50030; F2J10.19; Serine/threonine-protein kinase TOR; EC 2.7.11.1; Protein TARGET OF RAPAMYCIN; AtTOR
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential cell growth regulator that controls development from early embryo to seed production. Controls plant growth in environmental stress conditions. Acts through the phosphorylation of downstream effectors that are recruited by the binding partner RAPTOR. Acts by activating transcription, protein synthesis and ribosome biogenesis, and inhibiting mRNA degradation and autophagy. Can phosphorylate TAP46, a regulatory subunit of protein phosphatase 2A that modulates cell growth and survival. Involved in modulating the transition from heterotrophic to photoautotrophic growth by regulating the expression of chloroplast- and photosynthesis-associated genes. Essential for auxin signaling transduction, probably acting in polysomes to maintain the active ATPK1/S6K1 (and thus TIF3H1/eIF3h) phosphorylation status that is critical for translation reinitiation (e.g. uORF-mRNAs loading). Promotes abscisic acid (ABA) biosynthesis. Involved in the regulation of sugar-mediated (e.g. glucose and sucrose) glycolysis- and mitochondrial bioenergetics-dependent root growth promotion. Required for sugar (e.g. glucose) promotion of hypocotyl elongation in the dark, by activating the brassinosteroid pathway and stabilizing BZR1. The regulation of BZR1 degradation is dependent on autophagy. Regulates the expression, phosphorylation and ribosome association of MRFs (e.g. MRF1, MRF3 and MRF4), especially under energy-deficient conditions.; (Microbial infection) Binding to cauliflower mosaic virus (CaMV) Tav protein is critical for both translation reinitiation and viral fitness. When activated by CaMV P6, promotes CaMV translation by inhibiting cellular autophagy and suppressing both silencing and innate immunity, thus confering sensitivity to P.syringae.; (Microbial infection) Required during infection by some potyvirus such as Watermelon mosaic virus (WMV) but not for turnip mosaic virus (TuMV).
Gene References into Functions
  1. Sugar-activated TOR kinase phosphorylates and stabilizes E2Fa proteins to transcriptionally activate S-phase genes during callus formation. PMID: 28623850
  2. The dependence of potyviruses on active Arabidopsis TOR signalling was different. PMID: 25979731
  3. These findings advance the current understanding of rapamycin-TOR signaling in plants. PMID: 23989449
  4. highlight the possible role of TOR in carbon utilization and growth regulation in Arabidopsis PMID: 23761348
  5. TOR protein regulates growth and also influences nutrient partitioning and central energy metabolism. PMID: 23173928
  6. systems, cellular and genetic analyses uncover TOR phosphorylation of E2Fa transcription factor for an unconventional activation of S-phase genes, and glucose-signalling defects in e2fa root meristems PMID: 23542588
  7. Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. PMID: 22134914
  8. The authors show that the function of Cauliflower mosaic virus viroplasmin in reinitiation depends on physical association with TOR, with viroplasmin-TOR binding being critical for both translation reinitiation and viral fitness. PMID: 21343906
  9. TOR controls the embryogenesis, postembryonic development, and 45S rRNA production through its kinase domain in Arabidopsis. PMID: 21266656
  10. Results provide evidence that TOR is a negative regulator of autophagy in plants. PMID: 20686696
  11. The production of rapamycin-sensitive plants through the expression of baker's yeast FKBP12 protein illustrates the conservation of the TOR pathway in Arabidopsis. PMID: 17543119
  12. The AtTOR kinase is one of the contributors to the link between environmental cues and growth processes in plants. PMID: 17721444

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
PI3/PI4-kinase family
Tissue Specificity
Highly expressed in root meristems, shoot apical meristem (SAM) and floral buds.
Database Links

KEGG: ath:AT1G50030

STRING: 3702.AT1G50030.1

UniGene: At.16944

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