Recombinant Arabidopsis thaliana Transcription factor MYB44 (MYB44)

Code CSB-YP863724DOA
MSDS
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Source Yeast
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Code CSB-EP863724DOA
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Source E.coli
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Code CSB-EP863724DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863724DOA
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Source Baculovirus
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Code CSB-MP863724DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYB44
Uniprot No.
Alternative Names
MYB44; MYBR1; At5g67300; K8K14.2; Transcription factor MYB44; Myb-related protein 44; AtMYB44; Myb-related protein R1; AtMYBR1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-305
Target Protein Sequence
MADRIKGPWS PEEDEQLRRL VVKYGPRNWT VISKSIPGRS GKSCRLRWCN QLSPQVEHRP FSAEEDETIA RAHAQFGNKW ATIARLLNGR TDNAVKNHWN STLKRKCGGY DHRGYDGSED HRPVKRSVSA GSPPVVTGLY MSPGSPTGSD VSDSSTIPIL PSVELFKPVP RPGAVVLPLP IETSSSSDDP PTSLSLSLPG ADVSEESNRS HESTNINNTT SSRHNHNNTV SFMPFSGGFR GAIEEMGKSF PGNGGEFMAV VQEMIKAEVR SYMTEMQRNN GGGFVGGFID NGMIPMSQIG VGRIE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor. Represses the expression of protein phosphatases 2C in response to abscisic acid (ABA). Confers resistance to abiotic stresses dependent of ABA. In response to auxin, activates the transcription of the auxin-responsive gene IAA19. The IAA19 transcription activation by MYB44 is enhanced by direct interaction between MYB44 and PYL8. Transcriptional activator of WRKY70 by direct binding to its promoter region, especially at 5'-TAACNG-3' and 5'-CNGTTA-3' symmetric motifs. Activates salicylic acid (SA)- mediated defenses and subsequent resistance to biotrophic pathogen P.syringae pv. tomato DC3000, but represses jasmonic acid (JA)-mediated defenses responses against the necrotrophic pathogen A.brassicicola.
Gene References into Functions
  1. salt stress induces the eviction of H2A.Z-containing nucleosomes from the AtMYB44 promoter region. PMID: 29649476
  2. AtMYB44 might participate in the 3OC6-HSL-mediated primary root growth via regulating the expression of cytokinin- and auxin-related genes. The data establish a genetic connection between the regulatory role of AtMYB44 in phytohormones-related gene expression and plant response to the bacterial QS signal. PMID: 27604593
  3. The authors herein revealed that the MYB44-mediated prevention of Pseudomonas syringae penetration through the stomata is one of the components responsible for Penicillium simplicissimum GP17-2-elicited induced systemic resistance. PMID: 27301421
  4. Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress. PMID: 24531138
  5. AtMYB44 is an integrator of cross-talk between salicylic acid and jasmonic acid in plant defense responses. PMID: 23603962
  6. the C-terminal side of the protein, but not the N-terminal side of the R2R3 domain, contributes to the functional activity and specificity of AtMYB44 through interactions with other regulators generated by each of a variety of stimuli. PMID: 23161171
  7. the transcriptional regulator MYB44 has a role in the control of seed germination in Arabidopsis thaliana. PMID: 22704933
  8. AtMYB44 gene activation appears to not be induced by any specific hormone PMID: 20016937
  9. Enhanced abiotic stress tolerance of transgenic Arabidopsis overexpressing AtMYB44 was conferred by reduced expression of genes encoding a group of serine/threonine protein phosphatases 2C. PMID: 18162593

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, stems, leaves, inflorescence, and flowers (including stamen, floral nectar, carpel, petal and sepal), mostly in vasculatures and stomata.
Database Links

KEGG: ath:AT5G67300

STRING: 3702.AT5G67300.1

UniGene: At.252

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