Recombinant Arabidopsis thaliana UDP-glycosyltransferase 84A2 (UGT84A2)

Code CSB-YP862905DOA
MSDS
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Source Yeast
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Code CSB-EP862905DOA
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Source E.coli
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Code CSB-EP862905DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862905DOA
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Source Baculovirus
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Code CSB-MP862905DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UGT84A2
Uniprot No.
Alternative Names
UGT84A2; BRT1; SGT; SGT1; At3g21560; MIL23.13UDP-glycosyltransferase 84A2; Protein BRIGHT TRICHOMES 1; Sinapate 1-glucosyltransferase 1; AtSGT1; EC 2.4.1.120
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-496
Target Protein Sequence
MELESSPPLP PHVMLVSFPG QGHVNPLLRL GKLLASKGLL ITFVTTESWG KKMRISNKIQ DRVLKPVGKG YLRYDFFDDG LPEDDEASRT NLTILRPHLE LVGKREIKNL VKRYKEVTKQ PVTCLINNPF VSWVCDVAED LQIPCAVLWV QSCACLAAYY YYHHNLVDFP TKTEPEIDVQ ISGMPLLKHD EIPSFIHPSS PHSALREVII DQIKRLHKTF SIFIDTFNSL EKDIIDHMST LSLPGVIRPL GPLYKMAKTV AYDVVKVNIS EPTDPCMEWL DSQPVSSVVY ISFGTVAYLK QEQIDEIAYG VLNADVTFLW VIRQQELGFN KEKHVLPEEV KGKGKIVEWC SQEKVLSHPS VACFVTHCGW NSTMEAVSSG VPTVCFPQWG DQVTDAVYMI DVWKTGVRLS RGEAEERLVP REEVAERLRE VTKGEKAIEL KKNALKWKEE AEAAVARGGS SDRNLEKFVE KLGAKPVGKV QNGSHNHVLA GSIKSF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sinapate glucosyltransferase (SGT) required for the biosynthesis of the glucose ester sinapoylglucose and subsequently sinapoylmalate and sinapoylcholine. Is the major SGT activity in plant. Plays an important role in sinapoylation of anthocyanins. Sinapoylglucose produced by UGT84A2 is a significant source of sinapoyl moieties for anthocyanins. Indole-3-butyric acid (IBA)-specific glucosyltransferase that catalyzes the glucosylation of the auxin IBA, but not indole-3-acetic acid (IAA). May be involved in flowering regulation through IBA-mediated transcriptional repression of the auxin-response factors ARF6 and ARF8 and downstream flowering pathway genes. Can glucosylate the phytotoxic xenobiotic compound 2,4,5-trichlorophenol (TCP).
Gene References into Functions
  1. Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis PMID: 29027578
  2. UDP-glucosyltransferase UGT84A2/bright trichomes 1 (BRT1) is a novel component of Arabidopsis mesophyll nonhost resistance to Phakopsora pachyrhizi. PMID: 23356583
  3. UGT84A2 plays a major role in sinapoylation of anthocyanin PMID: 21899608
Protein Families
UDP-glycosyltransferase family
Tissue Specificity
Expressed in roots, cotyledons, leaf veins and trichomes.
Database Links

KEGG: ath:AT3G21560

STRING: 3702.AT3G21560.1

UniGene: At.38036

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