Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD)

Code CSB-YP327911CWX
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP327911CWX-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327911CWX
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP327911CWX
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
4hbD
Uniprot No.
Alternative Names
4hbD; CKL_3014NAD-dependent 4-hydroxybutyrate dehydrogenase; 4HBD; EC 1.1.1.61
Species
Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
Expression Region
1-371
Target Protein Sequence
MKLLKLAPDV YKFDTAEEFM KYFKVGKGDF ILTNEFLYKP FLEKFNDGAD AVFQEKYGLG EPSDEMINNI IKDIGDKQYN RIIAVGGGSV IDIAKILSLK YTDDSLDLFE GKVPLVKNKE LIIVPTTCGT GSEVTNVSVA ELKRRHTKKG IASDELYATY AVLVPEFIKG LPYKFFVTSS VDALIHATEA YVSPNANPYT DMFSVKAMEL ILNGYMQMVE KGNDYRVEII EDFVIGSNYA GIAFGNAGVG AVHALSYPIG GNYHVPHGEA NYLFFTEIFK TYYEKNPNGK IKDVNKLLAG ILKCDESEAY DSLSQLLDKL LSRKPLREYG MKEEEIETFA DSVIEGQQRL LVNNYEPFSR EDIVNTYKKL Y
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Could you please quote this 4-hydroxybutyrate dehydrogenase in all expression systems?
I also request for the enzyme activity (unit) for this enzyme.
https://www.cusabio.com/index.php?m=search&c=index&a=init&dosubmit=1&catid=init&typeid=&siteid=1&q=4-hydroxybutyrate+dehydrogenase

A:
Thanks for your interest in our product. Our distributor will send you the price soon, and pls check the detail information as follows:
Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
CSB-YP327911CWX >> Yeast
CSB-EP327911CWX >> E.coli
CSB-BP327911CWX >> Baculovirus
CSB-MP327911CWX >> Mammalian cell
Expression region: 1-371aa; Full length.
Tag Information: EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged; YP: N-terminal 6xHis-tagged
Sequenece:

MKLLKLAPDVYKFDTAEEFMKYFKVGKGDFILTNEFLYKPFLEKFNDGADAVFQEKYGLGEPSDEMINNIIKDIGDKQYNRIIAVGGGSVIDIAKILSLKYTDDSLDLFEGKVPLVKNKELIIVPTTCGTGSEVTNVSVAELKRRHTKKGIASDELYATYAVLVPEFIKGLPYKFFVTSSVDALIHATEAYVSPNANPYTDMFSVKAMELILNGYMQMVEKGNDYRVEIIEDFVIGSNYAGIAFGNAGVGAVHALSYPIGGNYHVPHGEANYLFFTEIFKTYYEKNPNGKIKDVNKLLAGILKCDESEAYDSLSQLLDKLLSRKPLREYGMKEEEIETFADSVIEGQQRLLVNNYEPFSREDIVNTYKKLY


Sorry, as we haven't tested the activity, I'm afraid we can't guanrantee the activity of this enzyme.
However, as what we provide is the full length protein, and it's purified under very mild condition, so in theory, it should be active.
We recommend you to purchase a small size to have try. If you have any question during the use of this product, pls feel free to contact us.
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