The production of recombinant dog Trefoil factor 3 (TFF3) is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered. Additionally, proteins expressed in Yeast, E. coli, and Mammalian cells have been successfully developed. The product codes are: CSB-YP801125DO, CSB-EP801125DO, CSB-MP801125DO.
TFF3 is a bioactive peptide characterized by a trefoil motif structure formed by three intra-peptide disulfide bonds [1]. It is mainly secreted by intestinal goblet cells and plays a role in mucosal repair in the gastrointestinal tract [2]. TFF3 stimulates cell proliferation and survival in breast and colorectal cancers [3][4]. Additionally, TFF3 promotes angiogenesis in mammary carcinoma and supports corneal wound healing effects [5][6].
The TFF3 signaling activates STAT3 which is essential for its oncogenic transformation properties [3]. TFF3 can also increase the expression of genes such as CCND1 and BCL2, further contributing to its role in cancer progression [3]. TFF3 is elevated in colorectal cancer and promotes malignant behavior by activating the epithelial-mesenchymal transition (EMT) process [8]. Moreover, TFF3 interacts with the CD147 receptor to regulate colorectal cancer progression [7]. TFF3 deficiency affects the morphology of the intestines, highlighting its protective role in the gastrointestinal mucosa [9].
References:
[1] S. Liu, D. Roberts, B. Zhang, Y. Ren, L. Zhang, & Y. Wu, Trefoil factor 3 as an endocrine neuroprotective factor from the liver in experimental cerebral ischemia/reperfusion injury, Plos One, vol. 8, no. 10, p. e77732, 2013. https://doi.org/10.1371/journal.pone.0077732
[2] Y. Yang, Z. Lin, Q. Lin, W. Bei, & J. Guo, Pathological and therapeutic roles of bioactive peptide trefoil factor 3 in diverse diseases: recent progress and perspective, Cell Death and Disease, vol. 13, no. 1, 2022. https://doi.org/10.1038/s41419-022-04504-6
[3] V. Pandey, M. Zhang, M. You, W. Zhang, R. Chen, W. Zhanget al., Expression of two non-mutated genetic elements is sufficient to stimulate oncogenic transformation of human mammary epithelial cells, Cell Death and Disease, vol. 9, no. 12, 2018. https://doi.org/10.1038/s41419-018-1177-6
[4] R. Chen, Y. Chiou, Q. Chong, H. Poh, T. Tan, M. Zhanget al., Pharmacological inhibition of tff3 enhances sensitivity of cms4 colorectal carcinoma to 5-fluorouracil through inhibition of p44/42 mapk, International Journal of Molecular Sciences, vol. 20, no. 24, p. 6215, 2019. https://doi.org/10.3390/ijms20246215
[5] W. Lau, V. Pandey, X. Kong, X. Wang, Z. Wu, T. Zhuet al., Trefoil factor-3 (tff3) stimulates de novo angiogenesis in mammary carcinoma both directly and indirectly via il-8/cxcr2, Plos One, vol. 10, no. 11, p. e0141947, 2015. https://doi.org/10.1371/journal.pone.0141947
[6] U. Schulze, U. Hampel, S. Sel, L. Contreras-Ruiz, M. Schicht, J. Dieckowet al., Trefoil factor family peptide 3 (tff3) is upregulated under experimental conditions similar to dry eye disease and supports corneal wound healing effects in vitro, Investigative Opthalmology & Visual Science, vol. 55, no. 5, p. 3037, 2014. https://doi.org/10.1167/iovs.13-13423
[7] H. Cui, S. Wang, F. Song, C. Xu, G. Nan, Y. Zhaoet al., Cd147 receptor is essential for tff3-mediated signaling regulating colorectal cancer progression, Signal Transduction and Targeted Therapy, vol. 6, no. 1, 2021. https://doi.org/10.1038/s41392-021-00677-2
[8] A. Yusufu, P. Shayimu, R. Tuerdi, C. Fang, F. Wang, & H. Wang, Tff3 and tff1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the emt process, International Journal of Oncology, 2019. https://doi.org/10.3892/ijo.2019.4854
[9] K. Šešelja, Tff3 deficiency differentially affects the morphology of male and female intestines in a long-term high-fat-diet-fed mouse model, International Journal of Molecular Sciences, vol. 24, no. 22, p. 16342, 2023. https://doi.org/10.3390/ijms242216342
