Recombinant Drosophila melanogaster Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs), partial

Code CSB-YP010329DLU
MSDS
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Source Yeast
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Code CSB-EP010329DLU
MSDS
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Source E.coli
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Code CSB-EP010329DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010329DLU
MSDS
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Source Baculovirus
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Code CSB-MP010329DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hrs
Uniprot No.
Alternative Names
Hrs; CG2903; Hepatocyte growth factor-regulated tyrosine kinase substrate
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential role in endosome membrane invagination and formation of multivesicular bodies, MVBs. Required during gastrulation and appears to regulate early embryonic signaling pathways. Inhibits tyrosine kinase receptor signaling by promoting degradation of the tyrosine-phosphorylated, active receptor, potentially by sorting activated receptors into MVBs. The MVBs are then trafficked to the lysosome where their contents are degraded.
Gene References into Functions
  1. The present report is devoted to the clarification and detailing of the Hrs effect on D/V, as well as on A/P borders of the imaginal wing disc compartments in Drosophila melanogaster. PMID: 27169237
  2. Hrs is dispensable for cytokinesis. Finally, it was found that although Drosophila Hrs does not localize at acrosome, the other endosomal markers--Rab4, Rab7, and Rab11--are detected at the organelle. PMID: 26591063
  3. Drosophila epithelia lacking Hrs and Stam, accumulate Notch and Dome in endosomes, maintain normal apico-basal polarity and proliferation control and do not show ectopic Notch signaling activation. PMID: 24718108
  4. Hrs mediates Smo trafficking in the late endosome by not only promoting Smo ubiquitination but also blocking Smo phosphorylation. PMID: 24244405
  5. these data support a model in which Ubpy plays a dual role in both cargo deubiquitylation and the ESCRT-0 subunit Hrs stability during development. PMID: 24574010
  6. Hrs protein is involved in the formation of the D/V boundary of the wing imaginal disk and suggested a new role in wing vein refinement for the Hrs gene PMID: 23113331
  7. show that the endosomal proteins Myopic (Mop) and Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) are required for the activation of the Toll signaling pathway. PMID: 20404143
  8. PtdIns(3)P specifically induces the membrane penetration of the FYVE domain of HRS protein and increases it membrane resonance time causing local conformational changes PMID: 12006563
  9. intracellular accumulation of receptors in Hrs mutant cells could be due to defective sorting towards lysosomal degradation or to defective post-endocytic retention in endosomes PMID: 14608370

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Subcellular Location
Cytoplasm, cell cortex. Cytoplasm, perinuclear region. Note=Locates to vesicles present in the perinuclear regions of muscle cells and in the periphery of Garland cells of third-instar larvae.
Database Links

KEGG: dme:Dmel_CG2903

STRING: 7227.FBpp0088692

UniGene: Dm.274

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